液相色谱-串联质谱法测定犬血浆中左旋泮托拉唑钠的浓度及其毒代动力学研究

发布时间：2018-04-15 17:14

本文选题：左旋泮托拉唑钠 + 犬血浆　；参考：《中国药学杂志》2017年15期

【摘要】：目的建立液相色谱-串联质谱法测定犬血浆中左旋泮托拉唑钠的浓度并研究其毒代动力学。方法犬血浆样本以乙腈沉淀蛋白后,选用CHIRALCEL OJ-RH色谱柱(4.6 mm×150 mm,5μm);流动相为乙腈-水=28∶72,流速为0.6 m L·min~(-1) ,柱温为35℃;选用Agilent 6430三重四极杆串联质谱仪,离子化方式:电喷雾-正离子(API-ES),监测方式:MRM,左旋泮托拉唑监测离子对384.0/199.8,非那西丁监测离子对180.0/110.0,用作内标。结果本分析方法专属性良好;左旋泮托拉唑钠在50~30 000 ng·m L~(-1) 内线性关系符合要求;准确度均在85%~115%,精密度RSD在15%内。Beagle犬连续4周静脉注射给予注射用左旋泮托拉唑钠低、中、高(10、20、40 mg·kg~(-1) ·d~(-1) )3个剂量,左旋泮托拉唑钠动力学参数AUC_(0-4h)和ρ_(max)增长与剂量增长均呈正相关。首次和4周给药后测定无明显差异。结论本法经方法学验证,适用于犬血浆中左旋泮托拉唑钠浓度测定,可用于左旋泮托拉唑钠犬体内毒代动力学研究。
[Abstract]:Objective to establish the determination of the concentration of - pantoprazole sodium in dog plasma by liquid chromatography tandem mass spectrometry and study the toxicokinetics. Methods canine plasma samples with acetonitrile protein precipitation, using CHIRALCEL OJ-RH column (4.6 mm * 150 mm, 5 m); mobile phase of acetonitrile water =28: 72 the flow rate was 0.6 m L min~ (-1), the column temperature is 35 DEG C; the Agilent 6430 three triple quadrupole tandem mass spectrometer ionization mode: positive electrospray ionization (API-ES), monitoring: MRM, - pantoprazole monitoring ions on 384.0/199.8, phenacetin monitoring ions of 180.0/110.0, as in standard analysis method. Results the specificity is good; the - pantoprazole sodium in the 50~30 000 ng m L~ (-1) linear relationship with the requirements; the accuracy was in 85%~115%, the precision of RSD.Beagle in 15% dogs in 4 consecutive weeks of intravenous injection with levo pantoprazole sodium injection, (low, high 10,20,40 Mg (kg~ -1) - d~ (-1)) 3 doses, the - pantoprazole sodium kinetics parameters of AUC_ (0-4h) and P _ (max) growth and growth are positively correlated. The first dose and 4 weeks after drug administration were no significant difference. Conclusion this method is applicable to the determination of the method validation, the - pantoprazole sodium the concentration in dog plasma, can be used for the - pantoprazole sodium in dogs toxicikinetics.

【作者单位】：山东省药学科学院;
【分类号】：R965

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