齐墩果酸及衍生物与牛血清白蛋白相互作用的荧光光谱分析

发布时间：2018-04-18 00:05

本文选题：齐墩果酸 + 衍生物　；参考：《中国药科大学学报》2017年05期

【摘要】：通过化学修饰改善齐墩果酸与牛血清白蛋白的相互作用,从而提高两者之间的结合能力。以齐墩果酸为先导物,分别对其C-3位羟基和C-28位的羧基进行修饰,得到4种不同的衍生物,并用IR和~1H NMR进行结构表征。采用荧光光谱法,研究了齐墩果酸及4种衍生物与牛血清白蛋白的相互作用规律,以及温度和Cu~(2+),Co~(2+)等金属离子对两者之间的相互作用的影响。与齐墩果酸相比,4种衍生物与牛血清白蛋白的相互作用都有提高,其中衍生物OA_2与牛血清白蛋白的相互作用提高了近16倍。Cu~(2+)和Co~(2+)等金属离子能明显增强衍生物OA_2与牛血清白蛋白间的相互作用。化学修饰是提高齐墩果酸与BSA之间的相互作用的有效途径。
[Abstract]:The interaction between oleanolic acid and bovine serum albumin was improved by chemical modification.The C-3 hydroxyl group and C-28 carboxyl group of oleanolic acid were modified and four different derivatives were obtained and characterized by IR and 1H NMR.The interaction of oleanolic acid and four derivatives with bovine serum albumin (BSA) and the effects of temperature and metal ions on the interaction between Oleanolic acid and four derivatives were studied by fluorescence spectrometry.Compared with oleanolic acid, the interaction of four derivatives with bovine serum albumin was increased.Metal ions such as derivative OA_2 and bovine serum albumin (BSA) can enhance the interaction between bovine serum albumin (BSA) and bovine serum albumin (BSA) by nearly 16 times. The metal ions such as OA_2) and Co~(2) can enhance the interaction between BSA and BSA.Chemical modification is an effective way to improve the interaction between oleanolic acid and BSA.
【作者单位】：广西民族大学广西多糖材料与改性重点实验室培育基地/广西高校化学与生物转化过程新技术实验室;
【基金】：广西高校人才小高地建设创新团队计划资助项目(桂教人[2011]47号12) 国家级大学生创新创业训练计划资助项目(No.201610608013)~~
【分类号】：R914

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