适配体介导脂质体靶向递送siRNA的研究

发布时间：2018-04-18 05:34

本文选题：适配体 + siRNA　；参考：《中国生物工程杂志》2015年01期

【摘要】：为探讨适配体介导的脂质体靶向递送siRNA的可行性,采用前列腺癌细胞膜表面抗原(PSMA)的适配体A10-3.2与脂质体结合,构建适配体-脂质体靶向递送体系(Apt-LP),并利用Apt-LP递送p EGFP-N1质粒和Bcl2 siRNA到前列腺癌细胞LNCa P(PSMA+)和PC-3(PSMA-),转染48h后,用荧光显微镜检测绿色荧光蛋白的表达,q PCR检测Bcl2 mRNA表达,蛋白印记法检测Bcl2蛋白表达,Hoechst 33258核染法分析体外抗肿瘤活性。结果显示:与脂质体递送体系相比,Apt-LP显著提高递送p EGFP-N1质粒和Bcl2 siRNA到靶细胞LNCa P(PSMA+)的效率;显著提高Bcl2 siRNA诱导的靶细胞LNCa P(PSMA+)Bcl2基因沉默效应,更有效的诱导靶细胞LNCa P(PSMA+)凋亡。结果表明:Apt-LP是一种有效的siRNA靶向递送体系,具有潜在的临床应用价值。
[Abstract]:In order to investigate the feasibility of aptamer mediated liposome targeting delivery of siRNA, the aptamer A10-3.2 of prostate cancer cell membrane surface antigen (PSMA) was used to bind to liposome.Aptamer liposome targeting delivery system (Apt-LPN) was constructed, and Apt-LP was used to deliver p EGFP-N1 plasmid and Bcl2 siRNA to prostate cancer cell line LNCa P(PSMA) and PC-3PSMA-G. After transfection for 48 h, the expression of green fluorescent protein (GFP) and Bcl2 mRNA were detected by fluorescence microscope.The expression of Bcl2 protein was detected by protein imprinting method. Hoechst 33258 nuclear staining method was used to analyze the anti-tumor activity in vitro.The results showed that compared with liposome delivery system, Apt-LP significantly increased the efficiency of delivering p EGFP-N1 plasmids and Bcl2 siRNA to target LNCa P(PSMA, increased the silencing effect of LNCa P(PSMA Bcl2 gene induced by Bcl2 siRNA, and more effectively induced LNCa P(PSMA apoptosis.The results show that: Apt-LP is an effective siRNA targeting delivery system and has potential clinical application value.
【作者单位】：兰州交通大学化学与生物工程学院;甘肃省医学科学研究院;
【基金】：甘肃省中青年基金(1107RJYA033) 兰州交通大学校青年基金(2013012)资助项目
【分类号】：R943

【共引文献】