不同浓度腐胺对人皮肤成纤维细胞增殖、迁移和凋亡的影响

发布时间：2018-04-19 23:13

本文选题：腐胺 + 增殖　；参考：《南方医科大学学报》2015年05期

【摘要】：目的探讨不同浓度腐胺对人皮肤成纤维细胞(human skin fibroblasts,HSF)增殖、迁移、凋亡的影响。方法将体外HSF分为腐胺组和对照组,以含腐胺浓度分别为0.5、1、5、10、50、100、500、1000μg/ml完全培养基培养细胞设为腐胺组,不添加腐胺设为对照组。经对照组及不同浓度腐胺组处理的HSF培养24 h后,再以四唑化合物电子耦联显色法(MTS法)测定细胞增殖能力(用吸光度值表示),Transwell迁移实验测定细胞的迁移能力,流式细胞技术(Flow Cytometry,FCM)Annexin V/PI标记法测定细胞凋亡率。结果 (1)细胞增殖能力(MTS)测定结果显示,0.5、1、5、10μg/ml腐胺组HSF的吸光度值较对照组升高(P0.01),并且1μg/ml时达峰值(0.754±0.024);500、1000μg/ml腐胺组HSF吸光度值较对照组降低(P0.01);50、100μg/ml腐胺组HSF的吸光度值与对照组无明显差异(P0.05);(2)Transwell迁移实验结果显示,1μg/ml腐胺组穿过微孔膜的细胞数目较对照组显著增加(P0.01),且达到峰值309±21;50μg/ml及以上浓度腐胺组HSF穿过微孔膜的数目较对照组减少(P0.05);0.5、5、10μg/ml腐胺组HSF穿过微孔膜的细胞数目与对照组比较无明显差异(P0.05);(3)流式细胞凋亡结果显示,0.5、1、5、10μg/ml腐胺组HSF凋亡率较对照组显著降低(P0.05),而且在1μg/ml时细胞凋亡率达最低值(11.10±0.79)%;100μg/ml及以上浓度腐胺组细胞凋亡率较对照组显著升高(P0.01),而且随着浓度增加凋亡率逐渐升高;50μg/ml腐胺组与对照组比较细胞凋亡率无明显差异(P0.05)。结论微量腐胺可维持细胞正常的迁移能力,显著提高HSF的细胞增殖能力,而较高浓度的腐胺能显著抑制HSF迁移与增殖,并诱导细胞发生凋亡,提示不同浓度的腐胺会对创面愈合起到完全不同的作用。
[Abstract]:Objective to investigate the effects of different concentrations of putrescine on the proliferation, migration and apoptosis of human skin fibroblast cells. Methods HSF in vitro was divided into putrescine group and control group. The cultured cells were divided into putrescine group and control group on 1 000 渭 g/ml culture medium containing putrescine. HSF treated with different concentrations of putrescine and control group were cultured for 24 h, and then the cell proliferation ability was measured by electron coupled chromogenic assay (MTS method). Flow Cytometry (FCM) Annexin V/PI labeling method was used to detect the apoptosis rate. Results the results of cell proliferation assay showed that the absorbance of HSF in 10 渭 g/ml putrescine group was higher than that in control group, and the peak value of HSF in 1 渭 g/ml group was 0.754 卤0.024 ~ 500 渭 g/ml putrescine, which was lower than that in control group (P0.0150100 渭 g/ml putrescine group) and control group (P 0.0150100 渭 g/ml putrescine group). The absorbance value of HSF in P0.0150100 渭 g/ml putrescine group was lower than that in control group. The results of Transwell migration test showed that the number of cells passing through the micropore membrane in the 1 渭 g/ml putrescine group was significantly higher than that in the control group, and the number of HSF passing through the micropore membrane reached a peak value of 309 卤21 50 渭 g/ml and more in the putrescine group than that in the control group. The number of HSF passing through the micropore membrane was less than that in the control group. There was no significant difference in the number of HSF cells passing through the micropore membrane compared with the control group. (P 0.05) the results of flow cytometry showed that the apoptosis rate of HSF in the group of 0. 5 渭 g/ml putrescine was significantly lower than that in the control group, and at 1 渭 g/ml the apoptotic rate reached the lowest value of 11. 10 卤0. 79100 渭 g/ml and above. The apoptotic rate of putrescine group was significantly higher than that of the control group (P 0.01), and the apoptosis rate increased gradually with the increase of concentration of 50 渭 g/ml putrescine compared with the control group. There was no significant difference in apoptosis rate between the two groups. Conclusion Trace putrescine can maintain the normal migration ability of the cells and significantly improve the proliferation of HSF cells, while the higher concentration of putrescine can significantly inhibit the migration and proliferation of HSF, and induce cell apoptosis. It suggested that different concentrations of putrescine could play a completely different role in wound healing.
【作者单位】：广州医科大学附属广州市第一人民医院烧伤外科;
【基金】：广州市重大民生专项(201300000100)
【分类号】：R96

【参考文献】