纳米银对皮肤细胞半通道活性的影响及其在细胞增殖抑制中的作用

发布时间：2018-04-20 03:22

  本文选题：纳米银 + 半通道　； 参考：《北京大学学报(医学版)》2017年03期

【摘要】：目的:探讨纳米银(silver nanoparticles,AgNPs)对皮肤细胞半通道活性的影响,并进一步研究半通道活性的改变在AgNPs诱导的细胞增殖抑制中的作用。方法:不同浓度(0、0.1、0.2、1.0、5.0、10.0、15.0和20.0μg/cm~2)的AgNPs处理人永生化表皮细胞系(HaCaT)24 h,采用碘化丙啶(propidium iodide,PI)染料排除法测定细胞活性,采用溴化乙锭(ethidium bromide,EB)摄取法测定细胞半通道活性。通过以常规密度(4×10~4个/cm~2)或低密度(1×10~4个/cm~2)接种细胞及采用半通道抑制剂甘珀酸(carbenoxolone,CBX)预处理的方法调节细胞半通道活性,采用细胞增殖-毒性检测试剂盒(cell counting kit-8,CCK-8)检测半通道活性受抑制、不同浓度AgNPs作用后的HaCaT细胞的增殖水平。结果:当AgNPs浓度低于10μg/cm~2时,HaCaT细胞的细胞活性未受明显影响。10μg/cm~2的AgNPs作用2、12和24 h可导致细胞半通道活性升高至对照组的116.67%、124.85%和139.53%,与对照组相比差异均具有统计学意义(P0.01),且25、50和100μmol/L CBX预处理可明显抑制10μg/cm~2AgNPs作用24 h后的细胞的半通道活性(P0.01)。此外,100μmol/L CBX预处理可升高低密度和常规密度下10μg/cm~2AgNPs作用24 h后细胞的增殖水平,其中,低密度下,CBX预处理组与AgNPs单独处理组相比,细胞增殖水平显著升高(P0.01)。结论:一定浓度的AgNPs可激活HaCaT细胞的半通道,半通道活性升高在AgNPs引发的细胞增殖抑制效应中具有重要作用。
[Abstract]:Aim: to investigate the effects of silver silver nanoparticlesclines (Ag NPs) on the half-channel activity of skin cells, and to investigate the role of the changes of half-channel activity in the inhibition of cell proliferation induced by AgNPs. Methods: human immortalized epidermal cell line HaCaT was treated with AgNPs at different concentrations of 0.1g / cm ~ (2) and 20.0 渭 g / cm ~ (-2) respectively. The cell activity was determined by propidium iodide PII dye exclusion method, and the half-channel activity was measured by ethidium bromidee uptake method. Cells were inoculated with conventional density of 4 脳 10 ~ 4 / cm ~ (-2) or low density of 1 脳 10 ~ (4) / cm ~ (-2) and treated with carbenoxolone (CBX), a half-channel inhibitor, to regulate the cell half-channel activity. Cell counting kit-8 CCK-8 was used to detect the inhibition of half-channel activity and the proliferation level of HaCaT cells treated with different concentrations of AgNPs. Results: when the concentration of AgNPs was lower than 10 渭 g / cm ~ 2, the cell activity of HaCaT cells was not significantly affected by AgNPs of 10 渭 g/cm~2 for 2 h and 24 h, and the half-channel activity increased to 116.67 ~ 124.85% and 139.53% of the control group. The difference was statistically significant compared with the control group. Pretreatment with 25 渭 mol/L and 100 渭 mol/L CBX significantly inhibited the half-channel activity of P0.01G after 24 h treatment with 10 渭 g/cm~2AgNPs. In addition, preconditioning with 100 渭 mol/L CBX could increase the proliferation level of cells treated with 10 渭 g/cm~2AgNPs at low density and normal density for 24 h, and the proliferation level of cells in low density pretreated group was significantly higher than that in AgNPs alone group. Conclusion: a certain concentration of AgNPs can activate the half-channel of HaCaT cells, and the increase of half-channel activity plays an important role in the inhibition of cell proliferation induced by AgNPs.
【作者单位】： 北京大学公共卫生学院劳动卫生与环境卫生学系;
【基金】：国家自然科学基金(21077006,21477004) 国家高技术研究发展计划(863计划,2012AA062804)资助~~
【分类号】：R99

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