表面印迹型脂质体靶向识别金黄色葡萄球菌的初步研究

发布时间：2018-04-21 00:12

本文选题：药剂学 + 表面印迹　；参考：《中国科技论文》2015年06期

【摘要】：为探索一种表面印迹型脂质体能靶向识别金黄色葡萄球菌的可行性,采用分子印迹技术制备表面印迹型脂质体,以表面修饰二氧化硅的脂质体为支撑基质,通过烯键接枝后,分别以丙烯酰胺为功能单体,N,N’-亚甲基双丙烯酰胺为交联剂,金黄色葡萄球菌表面蛋白(SPA)为模板分子开展表面印迹得到脂质体。脂质体的平均粒径为(200.5±60.5)nm,多分散系数为0.22±0.018,表观包封率平均值为(86.25±2.53)%。与非印迹型脂质体相比,印迹型脂质体表面具有良好的模板分子吸附性能与选择识别能力。包载荧光探针后并经流式细胞仪检测显示,印迹型脂质体对金黄色葡萄球菌的结合能力显著强于非印迹型脂质体,其荧光强度是后者的2.2倍;进一步包载药物后,印迹型脂质体能抑制细菌生长;可见印迹型脂质体具有深入研究的价值。
[Abstract]:In order to explore the feasibility of targeting staphylococcus aureus with surface imprinted liposomes, surface imprinted liposomes were prepared by molecular imprinting technique. The liposomes were imprinted on the surface of staphylococcus aureus surface protein (SPAA) using acrylamide as the functional monomer and methylene bisacrylamide as crosslinking agent and staphylococcus aureus surface protein spa as template molecule. The average diameter of liposome was 200.5 卤60.5 nm, the polydispersity coefficient was 0.22 卤0.018, and the average apparent entrapment efficiency was 86.25 卤2.53 nm. Compared with non-imprinted liposomes, imprinted liposomes have good adsorption performance and selective recognition ability on the surface of template molecules. The binding ability of imprinted liposomes to Staphylococcus aureus was significantly stronger than that of non-imprinted liposomes, and the fluorescence intensity of imprinted liposomes was 2.2 times higher than that of non-imprinted liposomes. Imprinted liposomes can inhibit bacterial growth.
【作者单位】：西南大学药学院;
【基金】：高等学校博士学科点专项科研基金资助项目(20110182120015)
【分类号】：R943

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