孕烯醇酮对大鼠空间学习记忆能力和ChAT蛋白表达的影响

发布时间：2018-04-21 01:31

本文选题：孕烯醇酮 + 神经甾体　；参考：《神经解剖学杂志》2015年02期

【摘要】：目的:研究孕烯醇酮(Pregnenolone,PREG)干预对老年雄性SD大鼠空间学习记忆能力的影响,探讨PREG的潜在神经保护作用及其机制。方法:24月龄雄性SD大鼠40只,随机分为对照组(A组)、溶剂对照组(B组)、低剂量PREG(0.5 mg/kg)干预组(C组)、高剂量PREG(2.0 mg/kg)干预组(D组),隔日腹腔注射一个月,利用Y迷宫训练评估PREG对实验大鼠空间学习记忆能力的影响,运用免疫荧光技术和Western Blot检测分析大鼠记忆相关脑区ChAT蛋白表达的变化。结果:Y迷宫训练结果显示D组大鼠平均连续正确反应次数为7.5±1.35,明显多于其他各组;免疫荧光检测结果显示D组大鼠前额叶、颞叶、海马内ChAT免疫阳性的细胞数量相应是26.8±2.6、19.8±2.6、25.8±2.4,明显多于其他处理组;Western Blot检测显示D组大鼠前额叶、颞叶、海马皮层组织ChAT蛋白的表达明显高于其他各组(P0.05)。结论:PREG有助于改善老年雄性SD大鼠空间学习记忆能力,其作用机制可能是促进了大鼠记忆相关脑区ChAT的表达上调,提高了胆碱能神经元细胞的功能活性。
[Abstract]:Aim: to study the effects of Pregnenolone preg on spatial learning and memory in aged male SD rats and to explore the potential neuroprotective effect of PREG and its mechanism. Methods Forty male Sprague-Dawley rats (24 months old) were randomly divided into three groups: control group A, solvent control group B, low dose PREG(0.5 mg / kg group C, high dose PREG(2.0 mg / kg group D, intraperitoneal injection for one month every other day. Y-maze training was used to evaluate the effect of PREG on the spatial learning and memory ability of experimental rats. The changes of ChAT protein expression in memory related brain regions of rats were detected by immunofluorescence and Western Blot. Results the average continuous correct reaction times of group D was 7.5 卤1.35, which was significantly higher than that of other groups, and the results of immunofluorescence showed that the prefrontal lobe and temporal lobe of D group were significantly higher than those of other groups. The expression of ChAT protein in prefrontal lobe, temporal lobe and hippocampal cortex in group D was significantly higher than that in group D (26.8 卤2.6 卤19.8 卤2.6 卤25.8 卤2.4), which was significantly higher than that in other groups. Conclusion: Preg can improve the spatial learning and memory ability of aged male SD rats, and its mechanism may be to promote the up-regulation of ChAT expression in memory related brain regions of rats and to increase the functional activity of cholinergic neurons.
【作者单位】：复旦大学附属金山医院神经内科;
【分类号】：R965

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