乙醇暴露对小鼠肝脏脂质合成的影响

发布时间：2018-04-21 08:40

本文选题：乙醇 + 肝细胞　；参考：《中华疾病控制杂志》2016年01期

【摘要】：目的观察小鼠不同时间乙醇暴露,其肝脏脂质合成情况。方法 8周龄雄性ICR小鼠随机分为空白对照组和乙醇实验组,实验组小鼠随机分为单次和连续4 d两个不同的时间组,均给予50%(V/V)乙醇溶液(4 g/kg体重)灌胃,对照组给予等体积的生理盐水。所有小鼠均于末次灌胃12 h后剖杀,取血清用于检测血液生化指标;取肝脏并称重,计算肝脏系数,检测甘油三酯(triglycerides,TG)和总胆固醇(total cholesterol,TCH)含量,苏木素伊红(hematoxylin and eosin,HE)染色观察其病理学改变,油红O染色观察其脂质沉积;反转录酶-聚合酶链锁反应(reverse transcription-polymerase chain reaction,RT-PCR)检测乙酰辅酶A羧化酶(acetyl coenzyme carboxylase,ACC)、脂肪酸合成酶(fatty acid synthase,FAS)和硬脂酰辅酶A去饱和酶-1(stearoyl coenzyme A desaturase-1,SCD-1)的mRNA水平。结果与对照组相比,单次或4 d乙醇暴露小鼠肝脏系数均增加(均有P0.05);血清和肝脏TG含量升高(均有P0.05);HE染色和油红O染色显示,肝脏发生脂质沉积;FAS、ACC和SCD-1 mRNA水平上调(均有P0.05)。结论单次或连续4 d乙醇暴露均可导致肝脏脂质沉积,并通过增加小鼠肝脏TG合成来完成。
[Abstract]:Objective to observe the lipid synthesis in liver of mice exposed to ethanol at different time. Methods 8-week-old male ICR mice were randomly divided into two groups: blank control group and ethanol experimental group. The experimental group mice were randomly divided into two groups: the control group and the control group. The mice in the experimental group were given 50 V / V ethanol solution for 4 g/kg. The control group was given saline of the same volume. All mice were dissected 12 hours after the last gavage, and the serum was taken for the determination of biochemical indexes in blood, the liver was taken and weighed, the liver coefficient was calculated, the contents of triglycerides triglycerides TGG) and total cholesterol total cholesterol total cholesterol (TC) were measured. The pathological changes were observed by hematoxylin and eosin hehe staining and lipid deposition was observed by oil red O staining. Reverse transcription-polymerase chain reactionation (RT-PCR) was used to detect the mRNA levels of acetyl coenzyme carboxylase1, fatty acid synthase fatty acid synthaseFASand stearoyl coenzyme A desaturase-1SCD-1. Results compared with the control group, the liver coefficients of mice exposed to ethanol at single or 4 days were all increased (P 0.05), and the serum and liver TG levels were increased (both P 0.05 he staining and oil red O staining showed that liver lipid deposition FASA ACC and SCD-1 mRNA levels were up-regulated (both P 0.05). Conclusion single or continuous ethanol exposure for 4 days can lead to lipids deposition in the liver, which can be accomplished by increasing the synthesis of TG in the liver of mice.
【作者单位】：安徽医科大学公共卫生学院营养与食品卫生学系;安徽医科大学公共卫生学院卫生毒理学系;
【基金】：国家自然科学基金(81402676) 安徽省高等学校省级自然科学研究项目(KJ2013A38) 安徽省自然科学基金(1508085QH187)
【分类号】：R96

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