16-妊娠双烯醇酮脂质体的体内外抗肿瘤作用研究

发布时间：2018-04-23 15:12

本文选题：-妊娠双烯醇酮 + 脂质体　；参考：《中国药学杂志》2015年14期

【摘要】：目的研究16-妊娠双烯醇酮脂质体的体内外抗肿瘤作用。方法采用噻唑蓝比色法测定16-妊娠双烯醇酮脂质体对多种人肿瘤细胞的生长抑制作用;建立人宫颈癌(He La)裸鼠移植模型,以空白脂质体为阴性对照组,顺铂为阳性对照组,16-妊娠双烯醇酮脂质体为实验组,经尾静脉注射给药,观察16-妊娠双烯醇酮脂质体的体内抗肿瘤作用。结果 16-妊娠双烯醇酮脂质体对人肝癌HepG2细胞,人口腔癌KB细胞,人乳腺癌T47D细胞,人胃癌SGC7901细胞,人成纤维肉瘤HT1080细胞,人卵巢癌SKOV3细胞,人前列腺癌PC3细胞,人前列腺癌DU145细胞,人肺癌A549细胞,人横纹肌肉瘤A204细胞和人宫颈癌HeLa细胞的IC50值分别为44.69,9.17,26.22,19.58,28.01,37.18,24.58,21.38,54.69,4.18和8.96μg·m L-1。尾静脉注射16-妊娠双烯醇酮脂质体7.5、15和30 mg·kg-1·d-1,28 d对人宫颈癌裸鼠移植瘤的相对肿瘤增殖率分别为93.7%、60.52%和37.84%,瘤重抑制率分别为23.05%、48.84%和69.70%。结论 16-妊娠双烯醇酮脂质体具有体内外抗肿瘤作用。
[Abstract]:Objective to study the anti-tumor effect of 16-gestational dienolone liposomes in vitro and in vivo. Methods the growth inhibition of 16-gestational dienolone liposomes on various human tumor cells was determined by thiazolyl blue colorimetry, and a nude mouse model of human cervical carcinoma was established, in which blank liposomes were used as negative control group. Cisplatin was used as the positive control group. The experimental group was treated with 16-gestational dienolone liposome. The in vivo anti-tumor effect of 16-gestational dienolone liposome was observed by intravenous injection of Cisplatin through tail vein. Results 16-gestational dienolone liposomes were used to treat human hepatoma HepG2 cells, human oral cancer KB cells, human breast cancer T47D cells, human gastric cancer SGC7901 cells, human fibrosarcoma HT1080 cells, human ovarian cancer SKOV3 cells, human prostate cancer PC3 cells. The IC50 values of human prostate cancer DU145 cells, human lung cancer A549 cells, human rhabdomyosarcoma A204 cells and human cervical cancer HeLa cells were 44.69 / 9.17 / 26.2219.58 / 28.01 and 21.34.69 / 4.18 and 8.96 渭 g / ml, respectively. The relative tumor proliferation rates were 93.70.52% and 37.84%, respectively, and the tumor weight inhibition rates were 23.05% and 69.70%, respectively. Conclusion 16-pregnancy dienolone liposomes have anti-tumor effect in vitro and in vivo.
【作者单位】：沈阳药科大学药学院;大连市药品检验所;
【基金】：辽宁省教育厅创新团队资助项目(LT2012018) 沈阳市科技局资助项目(F11-148-9-00) 辽宁特聘教授资助课题(辽教发[2014]187)
【分类号】：R965

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