固相萃取-HPLC法测定大鼠体内咖啡因、氨苯砜和氯唑沙宗的血药浓度及药动学研究

发布时间：2018-04-24 01:34

  本文选题：固相萃取 + 高效液相色谱法　； 参考：《中国药房》2015年34期

【摘要】：目的:测定大鼠体内咖啡因、氨苯砜和氯唑沙宗的血药浓度,计算药动学参数。方法:取大鼠6只,ig咖啡因、氨苯砜和氯唑沙宗混合溶液,给药剂量分别为1.5、2、3 mg/kg,于给药前及给药后0.5、1、2、3、4、6、8、12、24 h取血0.2~0.3 ml;血浆样品用固相萃取法处理,采用高效液相色谱法,以N-(2-羟乙基)邻苯二甲酰亚胺为内标,测定咖啡因、氨苯砜和氯唑沙宗的血药浓度;并用DAS 2.0软件计算药动学参数。结果:咖啡因、氨苯砜及氯唑沙宗检测质量浓度的线性范围均为0.2~30μg/ml(r分别为0.996 4、0.996 1、0.998 8),定量限均为0.2μg/ml,低、中、高质量浓度水平的方法回收率分别为(84.8±3.6)%~(111.4±10.2)%(RSD为4.3%~9.8%)、(107.0±13.3)%~(113.5±8.1)%(RSD为7.1%~14.0%)、(104.2±10.8)%~(111.1±12.2)%(RSD为8.0%~11.0%)(n=3);药动学参数tmax为(1.70±0.99)、(1.50±1.00)、(1.92±0.80)h,t1/2为(0.73±0.22)、(2.77±1.35)、(2.78±2.34)h,cmax为(2.60±0.50)、(5.78±1.19)、(9.76±1.37)mg/L,AUC0-t为(8.43±0.79)、(20.68±1.91)、(26.71±2.45)mg·h/L(n=6)。结论:本法操作简便、灵敏度高、结果准确,可用于咖啡因、氨苯砜和氯唑沙宗的血药浓度测定和药动学研究。
[Abstract]:Aim: to determine the plasma concentrations of caffeine, dapsone and chlorzoxazone in rats and calculate the pharmacokinetic parameters. Methods: a mixture of caffeine, dapsone and chlorzoxazone was taken from 6 rats. The dosage was 1.5 mg / kg. The blood samples were collected before and 24 hours after administration. The plasma samples were treated by solid phase extraction and HPLC, respectively. The plasma concentrations of caffeine, dapsone and chlorzoxazone were determined with N-chloride-2-hydroxyethyl phthalimide as internal standard, and the pharmacokinetic parameters were calculated by DAS 2.0 software. Results: the linear range of caffeine, dapsone and chlorzoxazone was 0.996 渭 g/ml(r and 0.998 8 渭 g/ml(r, respectively. The limit of quantification was 0.2 渭 g / ml. 楂樿川閲忔祿搴︽按骞崇殑鏂规硶鍥炴敹鐜囧垎鍒�涓�(84.8卤3.6)%~(111.4卤10.2)%(RSD涓，

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