吡格列酮对粥样动脉硬化模型的斑块稳定性及新生血管形成的作用

发布时间：2018-04-24 10:48

本文选题：吡格列酮 + 斑块稳定性　；参考：《中国临床药理学杂志》2017年12期

【摘要】：目的研究吡格列酮对粥样动脉硬化模型的斑块稳定性及新生血管形成的作用。方法选取新西兰白兔,按照体重随机分成实验组(20只)和模型组(20只);用高脂饲料喂养6周、普通的饲料喂养6周、高脂饲料再喂养4周的方法来建造动脉粥样的硬化模型;实验组,在高脂饲料开始的第1周,在高脂饲料里加入吡格列酮10 mg·kg~(-1),直到实验结束。在第8周和第18周,抽取血液样本,用生化试剂盒检测2组动物的高敏C反应蛋白(CRP)、血糖及血脂水平;用酶联免疫吸附试剂盒检测基质金属蛋白酶-9(MMP-9)水平。结果模型组与实验组巨噬细胞所占比例分别为(29.26±3.94)%,(8.78±4.01)%;这2组斑块的面积分别为(19.24±10.57)×10~(-2),(3.25±2.42)×10~(-2)mm~2;这2组新生血管数量分别为(163.03±72.96),(79.86±12.78)个,以上指标与模型组比较差异均有统计学意义(均P0.05)。动物18F-FDG摄取:模型组与实验组中期SUVmean比值分别为0.80±0.08,0.64±0.06;这2组晚期SUVmean比值分别为1.02±0.60,0.50±0.12;这2组中期SUVmax比值分别为1.00±0.07,0.84±0.06;这2组晚期SUVmax比值分别为1.28±0.12,0.61±0.08,实验组的这3种SUV与模型组比较差异均有统计学意义(均P0.05)。结论吡格列酮可减少斑块内部的炎症程度,加强斑块稳定性,减少新生血管的产生,减少易损斑块破损,从而起到抗动脉粥样硬化的作用。
[Abstract]:Objective to study the effects of pioglitazone on plaque stability and angiogenesis in atherosclerosis model. Methods New Zealand white rabbits were randomly divided into experimental group (n = 20) and model group (n = 20). In the experimental group, in the first week of high fat diet, pioglitazone was added to high fat diet with 10 mg / kg of pioglitazone-1, until the end of the experiment. At week 8 and week 18, blood samples were collected and the levels of Gao Min C-reactive protein (CRP), blood glucose and blood lipid were detected by biochemical kit, and the levels of matrix metalloproteinase-9 (MMP-9) were detected by enzyme-linked immunosorbent assay (Elisa). Results the percentage of macrophages in the model group and the experimental group was 29.26 卤3.94 and 8.78 卤4.01, respectively, and the plaque area of the two groups was 19.24 卤10.57 脳 10 ~ (-5) 卤2.42) 脳 10 ~ (10) ~ (-2) mm ~ (-2), and the number of neovascularization in these two groups was (163.03 卤72.96) 卤79.86 卤12.78, respectively, and there was significant difference between these two groups (P < 0.05). 18F-FDG uptake: the SUVmean ratio of the model group and the experimental group was 0.80 卤0.08 卤0.64 卤0.06, the late SUVmean ratio of the two groups was 1.02 卤0.60 卤0.50 卤0.12, the SUVmax ratio of the two groups was 1.00 卤0.07 卤0.84 卤0.06, the SUVmax ratio of the two groups was 1.28 卤0.120.61 卤0.08, respectively. The SUV of the experimental group and the model group were 1.28 卤0.120.61 卤0.08, respectively. The difference was statistically significant (P 0.05). Conclusion pioglitazone can reduce the degree of inflammation, strengthen the stability of plaque, reduce the formation of neovascularization, reduce the damage of vulnerable plaque, and thus play the role of anti-atherosclerosis.
【作者单位】：东营胜利石油管理局胜利医院心内科;山东大学齐鲁医院心血管内科;
【基金】：山东省自然科学基金资助项目(2014ZRE27448)
【分类号】：R965

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