激活脊髓小电导钙离子激活钾通道可抑制小鼠吗啡痛觉过敏

发布时间：2018-04-24 13:16

本文选题：SK通道 + -EBIO　；参考：《中国药理学通报》2017年04期

【摘要】：目的探究脊髓小电导钙离子激活钾通道(small conductance Ca~(2+)-activated K~+channels,SK通道)激活后对小鼠吗啡所致痛觉过敏的影响。方法选用♂C57BL6/N小鼠,建立吗啡痛觉过敏模型,鞘内注射SK通道激活剂1-EBIO后,测量小鼠热甩尾潜伏期(tail withdrawal latency,TWL),机械缩足阈值(mechanical withdrawal threshold,MWT)和内脏痛阈的变化。结果吗啡痛觉过敏模型小鼠与生理盐水对照组小鼠相比,热甩尾潜伏期、机械缩足阈值和内脏痛阈均降低;而鞘内注射SK通道激活剂1-EBIO后,与给药前相比,小鼠的痛阈热甩尾潜伏期,机械缩足阈值和内脏痛阈均升高;吗啡模型组小鼠的脊髓SK2膜蛋白表达量较对照组明显降低,而给予1-EBIO后,脊髓SK2膜蛋白表达量较模型组明显升高。结论脊髓SK通道参与小鼠吗啡引起的痛觉过敏。
[Abstract]:Objective to investigate the effect of small conductance Ca~(2 activated K ~ channel SK channel activation on morphine induced hyperalgesia in mice. Methods the morphine hyperalgesia model was established in C57BL6/N mice. After intrathecal injection of SK channel activator 1-EBIO, the changes of heat tail withdrawal latencyne TWLT, mechanical withdrawal threshold and visceral pain threshold were measured. Results compared with the control group, the latent period of heat flick, mechanical foot contraction threshold and visceral pain threshold in morphine hyperalgesia model mice were lower than those in normal saline control group, while after intrathecal injection of SK channel activator 1-EBIO, compared with those before administration, there was no significant difference between the two groups. The pain threshold heat flick latency, mechanical foot contraction threshold and visceral pain threshold increased in mice, and the expression of SK2 membrane protein in spinal cord in morphine model group was significantly lower than that in control group, but the expression of SK2 membrane protein in spinal cord was significantly higher than that in model group after administration of 1-EBIO. Conclusion SK channel in spinal cord is involved in morphine induced hyperalgesia in mice.
【作者单位】：徐州医科大学江苏省麻醉学重点实验室江苏省麻醉与镇痛应用技术重点实验室;安徽医科大学基础医学院;徐州医科大学附属医院急救中心;
【基金】：国家自然科学基金资助(No 81171041,81271217) 江苏省高校自然科学基金重点项目资助(No 13KJA320001) 江苏省自然科学基金资助(No BK20161171) 江苏省普通高校研究生科研创新计划项目资助(No KYLX16_1139)
【分类号】：R965

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