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UPLC法同时测定缬沙坦氢氯噻嗪片中缬沙坦和氢氯噻嗪的含量

发布时间:2018-04-27 17:33

  本文选题:超高效液相色谱法 + 缬沙坦氢氯噻嗪片 ; 参考:《中国药房》2017年15期


【摘要】:目的:建立同时测定缬沙坦氢氯噻嗪片中缬沙坦和氢氯噻嗪含量的方法。方法:采用超高效液相色谱法。色谱柱为Phenomenex C_(18),流动相为[0.1%磷酸溶液-乙腈(95∶5,V/V)]-[0.1%磷酸溶液-乙腈(5∶95,V/V)](梯度洗脱),流速为0.25 mL/min,检测波长为272 nm,柱温为35℃,进样量为1.5μL。结果:缬沙坦和氢氯噻嗪检测质量浓度线性范围分别为8.1~324.2μg/mL(r=0.999 9)、1.2~50.1μg/mL(r=0.999 9);定量限分别为0.24、0.04 ng,检测限分别为0.06、0.01 ng;精密度、稳定性、重复性试验的RSD2.0%;加样回收率分别为97.69%~100.35%(RSD=1.03%,n=9)、98.27%~100.60%(RSD=0.83%,n=9)。结论:该方法操作简单、快速,结果准确,可用于缬沙坦氢氯噻嗪片中缬沙坦和氢氯噻嗪含量的同时测定。
[Abstract]:Objective: to establish a method for simultaneous determination of valsartan and hydrochlorothiazide in valsartan hydrochlorothiazide tablets. Methods: ultrahigh performance liquid chromatography was used. The chromatographic column was Phenomenex Che 18, the mobile phase was [0.1% phosphoric acid solution-acetonitrile 95: 5 V / V]-[0.1% phosphoric acid solution-acetonitrile 5: 95 V / V] (gradient elution, flow rate was 0.25 mL / min, detection wavelength was 272nm, column temperature was 35 鈩,

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