基于Cole-Cole模型评价纳米二氧化钛的细胞毒性作用

发布时间：2018-04-28 01:26

本文选题：纳米二氧化钛 + 胃癌　；参考：《中国生物医学工程学报》2017年03期

【摘要】：利用Cole-Cole模型评价不同剂量纳米二氧化钛(Nano-TiO_2)的细胞毒性作用,并探讨电生理机制。150和300 mg/L的Nano-TiO_2悬液作用人胃癌MGC803细胞24 h后,制备成细胞悬浮液。在1 k Hz~100 MHz范围,采用Agilent 4294A精密阻抗分析仪测量了人胃癌MGC803细胞悬浮液电阻抗的幅值和相位角,经电阻抗频谱和Nyquist图的曲线拟合的残差分析,建立Cole-Cole模型参数,评估Nano-TiO_2对MGC803细胞导电性能的影响。结果表明,150和300 mg/L的Nano-TiO_2引起第一电阻抗增量(ΔZ1)分别减少18.18%(P0.001)和39.39%(P0.001),第二电阻抗增量(ΔZ2)分别减少6.56%(P0.001)、8.2%(P0.001),降低了MGC803细胞膜及其核膜的电阻,增加了其导电性能;第一特征频率(fC1)分别增加19.74%(P0.001)和29.67%(P0.001),第二特征频率(fC2)分别增加6.28%(P0.001)、23.43%(P0.001);第一散射角(β1)分别降低1.35%(P0.05)和2.70%(P0.05)。ColeCole模型可评价Nano-TiO_2的细胞毒性作用并解释其电生理机制,为纳米颗粒的细胞毒理研究提供一种电特性方法。
[Abstract]:Cole-Cole model was used to evaluate the cytotoxicity of different doses of Nano-TiO-2 (Nano-TiO-2), and to investigate the electrophysiological mechanism. 150 mg/L and 300 mg/L Nano-TiO_2 suspension were used to treat MGC803 cells for 24 h, and then the suspension of human gastric cancer MGC803 cells was prepared. In the range of 1 k Hz~100 MHz, the amplitude and phase angle of suspension impedance of human gastric cancer MGC803 cells were measured by Agilent 4294A precise impedance analyzer. The parameters of Cole-Cole model were established by residual analysis of impedance spectrum and Nyquist curve fitting. To evaluate the effect of Nano-TiO_2 on the electrical conductivity of MGC803 cells. The results showed that the first electrical impedance increment (螖 Z _ 1) and the second impedance increment (螖 Z _ 2) were reduced by 18.18 and 39.39, respectively, and the second impedance increment (螖 Z _ 2) decreased by 6.56 ~ (-1) P _ (0.001) (P _ (0.001) (P _ (0.001), respectively, which decreased the resistance of MGC803 cell membrane and its nuclear membrane, and increased its electrical conductivity. The first characteristic frequency (fC1) and the second characteristic frequency (FC2) increased by 19.74g / P0.001) and 29.67kb / P0.001a, respectively, and the second characteristic frequency (fC2) increased by 6.28 and 23.43p respectively, and the first scattering angle (尾 _ 1) decreased by 1.35g / P0.05) and the 2.70%(P0.05).ColeCole model could be used to evaluate the cytotoxicity of Nano-TiO_2 and explain its electrophysiological mechanism. To provide a method for the study of cellular toxicology of nanoparticles.
【作者单位】：宁波大学医学院;
【基金】：国家自然科学基金项目(51277099) 宁波市科技惠民项目(2015C5009)
【分类号】：R99

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