RP-HPLC法同时测定人血浆中丙戊酸钠及其代谢产物的浓度

发布时间：2018-05-01 02:33

  本文选题：丙戌酸钠 + -丙基--戌烯酸　； 参考：《中国药房》2017年05期

【摘要】：目的:建立同时测定人血浆中丙戊酸钠(VPA)及其代谢产物2-丙基-2-戊烯酸(2-ene-VPA)浓度的方法。方法:血浆样品经环己烷沉淀蛋白后,用2,4'-二溴苯乙酮进行衍生化,以正辛酸为内标,采用反相高效液相色谱法测定。色谱柱为Zorbax SB-C_(18),流动相为乙腈-水(65:35,V/V),流速为1 mL/min,柱温为35℃,紫外检测波长为258 nm,进样量为20μL。结果:VPA、2-ene-VPA血药浓度分别在5.0~200.0、0.5~20.0μg/mL范围内线性关系良好(r均为0.999 9,n=5),定量下限分别为5.0、0.5μg/mL;日内、日间RSD均小于5%,方法回收率分别为95.99%~98.80%、97.40%~98.17%,提取回收率分别为80.46%~86.23%、80.45%~85.61%。采用该法测得10例癫痫患儿VPA血药浓度为27.4~93.2μg/mL,2-ene-VPA血药浓度为0.85~3.94μg/mL。结论:该方法操作简便、专属性强,可用于VPA血药浓度检测及药动学研究。
[Abstract]:Aim: to establish a method for simultaneous determination of valproate sodium valproate and its metabolite 2-propyl-2-pentenene-VPA in human plasma. Methods: the plasma samples were precipitated by cyclohexane and then derivatized with 2-dibromoacetophenone. N-octanoic acid was used as the internal standard and was determined by reversed-phase high performance liquid chromatography (RP-HPLC). The chromatographic column was Zorbax SB-CN 18, the mobile phase was acetonitrile-water 65: 35 V / V / V, the flow rate was 1 mL / min, the column temperature was 35 鈩，

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