氯化镨对小鼠骨髓细胞DNA的损伤及硒的干预作用

发布时间：2018-05-02 18:50

本文选题：氯化镨 + 亚硒酸钠　；参考：《毒理学杂志》2017年04期

【摘要】：目的观察氯化镨(Pr Cl3)对小鼠骨髓细胞DNA的损伤,以及亚硒酸钠(Na_2SeO_3)的干预作用。方法试验动物为5~6周龄昆明种小鼠;试验设置12个组,即5个Pr Cl_3剂量组(7.5、15、30、60和120 mg/kg·bw)、5个Na_2SeO_3干预组(在5个PrCl3剂量的基础上分别加入0.05 mg/kg·bw的Na_2SeO_3)、2个对照组[阴性组为0.85%生理盐水,阳性组为叠氮化钠(Na N3)20 mg/kg·bw]。各组小鼠采用腹腔注射染毒,运用单细胞凝胶电泳技术研究各组小鼠DNA受损情况,并比较Na_2SeO_3干预组对小鼠骨髓细胞DNA的影响。结果在7.5~120 mg/kg·bw的PrCl_3剂量内,随着PrCl_3处理剂量的增加,头长先增加后减少,尾长(TL)、全长(CL)、尾部DNA百分含量(TDNA)、尾矩(TM)、Olive尾矩(OTM)和拖尾率(PTC)逐渐增加,且存在明显的剂量-效应关系(r_(TL)=0.95,r_(CL)=0.88,r_(TDNA)=0.99,r_(TM)=0.99,r_(OTM)=0.97,r_(PTC)=0.95);将Na_2SeO_3干预组与PrCl_3剂量组两两对应相比较,Na_2SeO_3干预组的小鼠骨髓细胞尾长、全长、尾部DNA百分含量、尾矩、Olive尾矩和拖尾率均降低,经t检验显示,除7.5和15 mg/kg·bw组尾部DNA百分含量差异无统计学意义以外,其余各组各指标差异均有统计学意义(P0.05或P0.01)。结论在实验浓度范围内PrCl_3致小鼠骨髓细胞核DNA损伤逐渐增强,具有遗传毒作用;加入Na_2SeO_3能够降低小鼠骨髓细胞核DNA的损伤,具有明显的干预作用。
[Abstract]:Objective to observe the effect of praseodymium chloride (pr Cl 3) on DNA damage in bone marrow cells of mice and the intervention of sodium selenite (Na2SeO 3). Methods the mice were divided into 12 groups, 5 pr Cl_3 dosage groups, 60 and 120 mg/kg BWN groups, 5 Na_2SeO_3 intervention groups (0. 05 mg/kg BW Na2SeOStax, 0. 05 mg/kg BW), and 2 control groups (negative group, 0. 85% normal saline), and 5 Na_2SeO_3 intervention groups (0. 05 mg/kg BW, 0. 85% normal saline), 5 Na_2SeO_3 intervention groups (0. 05 mg/kg BW, 0. 85% normal saline, 0. 85% normal saline respectively). The positive group was sodium azide sodium nitride for 20 mg/kg BW. Single cell gel electrophoresis (SCGE) was used to study the damage of DNA in mice in each group, and to compare the effect of Na_2SeO_3 intervention group on DNA in bone marrow cells of mice. Results with the increase of PrCl_3 treatment dose, the head length increased first and then decreased with the increase of PrCl_3 dose of 7.5 ~ 120 mg/kg BW. The tail length increased and then decreased, and the tail length and tail length increased gradually. The percentage of DNA in the tail was increased with the increase of DNA content of the tail, and the tail length was increased with the increase of the tail length and tail rate (TLC), and the tail length increased gradually with the increase of the PrCl_3 dose at the dose of 7.5 ~ 120 mg/kg BW. And there is a significant dose-effect relationship between the Na_2SeO_3 intervention group and the Na2SeO3 intervention group. The tail length, tail DNA content, tail Olive tail moment and tail towing rate of the Na_2SeO_3 intervention group and the Na2SeO3 intervention group are all decreased, and the tail length, total length, tail DNA content, tail Olive tail moment and tail towing rate of the Na2SeO3 intervention group are all decreased, and the tail length, the total length, the tail DNA content, the tail Olive tail moment and the tail dragging rate of the Na2SeO3 intervention group are all decreased, the results of t test show that the bone marrow cells in the Na_2SeO_3 intervention group and the Na2SeO3 intervention group have lower tail length, full length, tail DNA content, tail Olive tail moment and tail towing rate. Except for 7.5 mg/kg BW group and 15 mg/kg BW group, there was no significant difference in tail DNA percentage, but there were significant differences in other indexes (P0.05 or P0.01). Conclusion in the range of experimental concentration of PrCl_3, the damage of DNA in bone marrow nucleus of mice was gradually enhanced, and Na_2SeO_3 could reduce the damage of DNA in bone marrow nucleus of mice, and had obvious intervention effect.
【作者单位】：徐州工程学院环境工程学院;江苏师范大学生命科学学院;
【基金】：国家自然科学基金(30470421) 江苏省高校自然科学基金(08KJD180010)
【分类号】：R99

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