尤瑞克林对脑缺血再灌注大鼠微血管新生的影响

发布时间：2018-05-02 22:12

本文选题：尤瑞克林 + 脑缺血再灌注　；参考：《中国临床药理学杂志》2017年21期

【摘要】：目的观察尤瑞克林对大鼠脑缺血再灌注损伤后缺血半暗带区域脑组织内微血管新生及血管生成素-1(Ang-1)及酪氨酸激酶受体-2(Tie-2)表达的影响。方法按照体重将大鼠随机分为3组:假手术组、模型组和实验组。采用线栓法制作大脑中动脉阻塞脑缺血再灌注模型。于再灌注后5 min,实验组静脉注射3.5×10~(-3)PNAU·kg~(-1)尤瑞克林1 mL·kg~(-1);假手术组和模型组正常喂养。于再灌注48 h后,断头取脑组织,用免疫组织荧光染色法检测缺血半暗带区域脑组织内微血管计数,用半定量RT-PCR法及免疫印迹法检测缺血半暗带区域脑组织内Ang-1与Tie-2的表达。结果给药后,模型组与实验组脑组织中微血管计数分别为23.51±1.94,37.41±2.51;这2组的Ang-1 mRNA表达分别为0.22±0.01,0.31±0.02;这2组的Ang-1蛋白表达分别为0.26±0.02,0.41±0.03;这2组的Tie-2 mRNA表达分别为0.27±0.02,0.42±0.03;这2组的Tie-2蛋白表达分别为0.53±0.04,0.71±0.07。与模型组比较,各个指标差异均有统计学意义(均P0.05)。结论尤瑞克林可促进大鼠脑缺血后微血管新生,其机制可能与上调Ang-1、Tie-2的表达有关。
[Abstract]:Objective to investigate the effects of eurexine on the expression of angiopoietin 1 (Ang-1) and tyrosine kinase receptor-2 (Tie-2) in the brain tissue of the ischemic penumbra after cerebral ischemia reperfusion injury in rats. Methods according to body weight, rats were randomly divided into 3 groups: sham operation group, model group and experimental group. The middle cerebral artery occlusion model of cerebral ischemia reperfusion was established by thread occlusion. At 5 minutes after reperfusion, the experimental group was injected intravenously with 3. 5 脳 10~(-3)PNAU kg-1) urelin 1 mL kg-1, which was fed normally in the sham operation group and the model group. After 48 hours of reperfusion, the brain tissue was cut off, and the microvessel count in the ischemic penumbra was detected by immunofluorescence staining, and the expression of Ang-1 and Tie-2 in the ischemic penumbra was detected by semi-quantitative RT-PCR and Western blotting. Results after administration, the microvessel count in the brain tissue of the model group and the experimental group was 23.51 卤1.94 卤37.41 卤2.51respectively, the Ang-1 mRNA expression of the two groups was 0.22 卤0.01U 0.31 卤0.02, the expression of Ang-1 protein was 0.26 卤0.02n0.41 卤0.03in the two groups, the Tie-2 mRNA expression in the two groups was 0.27 卤0.02n0.42 卤0.03respectively, and the expression of Tie-2 protein was 0.53 卤0.040.71 卤0.07in the two groups. Compared with the model group, the differences of each index were statistically significant (P 0.05). Conclusion Urestrin can promote microangiogenesis after cerebral ischemia in rats, and its mechanism may be related to the up-regulation of Ang-1 tie-2 expression.
【作者单位】：福建医科大学附属泉州第一医院神经内科;
【基金】：福建省卫生计生委青年科研课题基金资助项目(2014-1-85) 泉州市科技计划基金资助项目(2013Z59)
【分类号】：R965

【相似文献】