马铃薯淀粉纳米颗粒的制备与特性表征及其作为药物载体材料缓释性的研究

发布时间：2018-05-04 00:43

本文选题：超声波辅助酸解 + 淀粉纳米颗粒　；参考：《甘肃农业大学》2017年硕士论文

【摘要】：本论文以马铃薯淀粉为原料,用超声波辅助酸解法制备淀粉纳米颗粒,研究了超声功率、硫酸浓度和酸解时间对颗粒粒径及产率的影响,得出最佳制备工艺参数,并对制备的淀粉纳米颗粒的理化性质进行表征;以制备的淀粉纳米颗粒为载体材料,湿法制粒得到5-氨基水杨酸的药物片芯,研究了片芯在模拟人工胃肠道环境中的缓释效果;对缓释片芯经过肠溶包衣后,研究了包衣片在模拟胃肠道环境中药物的累积释放百分率,并对其进行体外释药动力学模型拟合;以大肠杆菌为指示菌,通过测量抑菌圈的大小,验证了不同时间点5-氨基水杨酸缓释包衣片的药物释放程度,研究结果如下:1.用超声波对原淀粉进行预处理后再进行酸水解,分析所得产物的粒径、产率得出最佳制备工艺参数为:在40℃条件下,用3 mol/L硫酸水解15%的淀粉乳,搅拌速度为100 r/min,超声功率400 W,酸解20 h后,得到的淀粉纳米颗粒平均粒径在50~80 nm范围内,产率为14.1%。2.将原淀粉与最佳工艺参数制备的淀粉纳米颗粒相比,结晶度由21.57%增长到46.35%,吸水率由34.8%增长到96.9%,吸附率由49.1%增长到93.7%,比表面积由0.1789 m2/g增长到1.6491 m2/g,氮气吸附量由0.68374 cm3/g增长到1.34734 cm3/g,说明淀粉纳米颗粒具有强大的表面能和吸附性能。3.以5-ASA为主药,分别用原淀粉和淀粉纳米颗粒为载体材料,湿法制粒得到片芯,在模拟人工胃肠液中用透析法进行释放试验,统计不同释放介质中的累积释药百分率。结果表明:以淀粉纳米颗粒为载体材料片芯的累计释药百分率均低于以原淀粉为载体材料的片芯,因此,纳米淀粉具有明显的缓释效果。4.对缓释片芯进行肠溶包衣后得到缓释包衣片,将其置于模拟人工胃肠环境中,统计不同释放介质中药物的累积释药百分率,对药物释放率进行模型拟合。结果表明:缓释包衣片在模拟人工胃肠环境中的累积释药百分率与释药时间的拟合模型呈对数形式的缓慢释放,符合缓释片剂非恒速释放的一级释药动力学模型。5.以大肠菌为指示菌,用抑菌试验去验证缓释包衣片体外释放试验后药物的释放程度。结果表明:缓释包衣片在模拟胃肠环境中的累积释药率持续增大,在反应15 h后累积释药率达到87.1%,抑菌圈为25.67 mm,说明药物在结肠液中大量释放,发挥了药物在结肠的药效。
[Abstract]:This paper uses potato starch as raw material to prepare starch nanoparticles by ultrasonic assisted acid hydrolysis. The effects of ultrasonic power, sulfuric acid concentration and acid hydrolysis time on particle size and yield are studied. The optimum processing parameters are obtained. The physicochemical properties of the prepared starch nanoparticles are characterized. The prepared starch nanoparticles are prepared. The drug core of 5- amino salicylic acid was obtained by wet granulation. The release effect of the core in the simulated artificial gastrointestinal environment was studied. The cumulative release percentage of the drugs in the simulated gastrointestinal tract was studied after the release of the release core in the intestinal tract. By measuring the size of bacteriostasis, the degree of drug release of 5- amino salicylic acid sustained-release coated tablets at different time points was verified. The results were as follows: 1. the original starch was pretreated with ultrasonic wave after acid hydrolysis, and the particle size of the product was analyzed. The best preparation parameters were obtained at 40 degrees centigrade. 3 mol/L sulfuric acid hydrolyzed 15% starch milk, stirring speed was 100 r/min, ultrasonic power was 400 W, and acid solution 20 h, the average particle size of starch nanoparticles was within 50~80 nm range. The yield was 14.1%.2., the crystallinity increased from 21.57% to 46.35%, and the water absorption rate increased from 34.8% to 96., compared with the starch nanoparticles prepared by the optimum process parameters. 9%, the adsorption rate increased from 49.1% to 93.7%, the specific surface area increased from 0.1789 m2/g to 1.6491 m2/g, and the nitrogen adsorption capacity increased from 0.68374 cm3/g to 1.34734 cm3/g, indicating that the starch nanoparticles have strong surface energy and adsorption property.3. with 5-ASA as the main drug, respectively, using the raw starch and amylum nanoparticles as the carrier material, and the wet granulation to get the chip core. In the simulated artificial gastrointestinal fluid, the release test was carried out by dialysis, and the cumulative release percentage in different release media was calculated. The results showed that the cumulative release percentage of the core with starch nanoparticles as the carrier was lower than that of the raw starch as the carrier material. Therefore, the nanoscale starch had a significant release effect of.4. to the sustained release tablets. The core was coated with enteric coated coated tablets and placed in the simulated artificial gastrointestinal environment. The cumulative release percentage of drugs in different release media was calculated and the drug release rate was fitted. The results showed that the fitting model of the cumulative release rate of the sustained release coating tablets in the simulated artificial gastrointestinal environment and the time of drug release was on the basis of the fitting model. The slow release of several forms was in line with the first order release kinetics model of the sustained release tablets,.5., with Escherichia coli as the indicator. The release degree of the drug released after the release test in vitro was verified by the bacteriostasis test. The results showed that the cumulative release rate of the sustained-release coated tablets in the simulated gastrointestinal environment increased continuously, after the reaction of 15 h. The cumulative release rate reached 87.1% and the inhibition zone was 25.67 mm, indicating that the drug was released in colon juice and played a role in the colon.

【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R943

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