不同剂量阿霉素对小鼠心脏结构与功能的影响

发布时间：2018-05-04 06:16

本文选题：阿霉素 + 心脏结构　；参考：《中国地方病防治杂志》2017年03期

【摘要】：目的观察不同剂量阿霉素致小鼠心肌的损伤作用。方法 32只小鼠随机分为健康对照和低、中、高剂量阿霉素组,每组8只,低、中、高剂量阿霉素组分别以1.5、3.0、4.5 mg/kg剂量隔日1次腹腔注射阿霉素,共7次,对照组腹腔注射等体积生理盐水。末次注射阿霉素后72h,采集动脉血测血清中LDH、CK、CK-MB含量,取心肌组织切片观察心肌结构变化。结果与正常对照组、低、中剂量阿霉素组比较,高剂量阿霉素组血清LDH、CK、CK-MB明显升高(P0.05),光镜下可见心肌细胞内大量空泡变性,细胞间隙明显增宽,伴大量炎细胞浸润,间质内可见毛细血管扩展,胶原纤维含量增多。结论 4.5 mg/kg阿霉素为诱导小鼠心肌明显损伤的最适剂量。
[Abstract]:Objective to observe the effects of different doses of adriamycin on myocardial injury in mice. Methods Thirty-two mice were randomly divided into healthy control group and low dose group, middle and high dose adriamycin group (n = 8 in each group), middle and high dose adriamycin group were intraperitoneally injected adriamycin (adriamycin) once every other day at a dose of 1.5 ~ 3.04.5 mg/kg for a total of 7 times. The control group was intraperitoneally injected with normal saline of the same volume. At 72 hours after the last injection of adriamycin, blood samples were collected to measure the level of CK-MB in serum, and myocardial tissue sections were taken to observe the changes of myocardial structure. Results compared with the normal control group, low and middle dose adriamycin group, the serum LDHN CKK-MB in the high dose adriamycin group was significantly increased (P 0.05). Under the light microscope, a large number of vacuolar degeneration in cardiomyocytes, widening of the intercellular space and infiltration of a large number of inflammatory cells were observed. Capillary dilation and collagen fiber content were observed in the interstitial tissue. Conclusion 4.5 mg/kg doxorubicin is the best dose for inducing obvious myocardial injury in mice.
【作者单位】：承德医学院附属医院;解放军昆明总医院干细胞与组织器官工程研究中心;
【基金】：承德市科学技术研究与发展计划项目(编号:201601A055)
【分类号】：R965

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