替普瑞酮对胃上皮细胞增殖、迁移及凋亡的影响

发布时间：2018-05-06 06:42

本文选题：胃上皮细胞 + 替普瑞酮　；参考：《重庆医学》2015年16期

【摘要】：目的观察替普瑞酮对人正常胃上皮细胞增殖、迁移及凋亡等生物学现象的影响。方法体外培养人正常胃上皮细胞,MTT法检测不同浓度替普瑞酮对胃上皮细胞的增殖作用并确定替普瑞酮最佳作用浓度。Transwell实验、划痕实验检测替普瑞酮最佳作用浓度对胃上皮细胞运动能力及迁移作用的影响。流式细胞法检测替普瑞酮最佳作用浓度对胃上皮细胞凋亡的影响。结果替普瑞酮作用于胃上皮细胞24h后,开始出现促进胃上皮细胞增殖的现象,从10~80μmol/L随着浓度增加,促进作用越明显,而80~320μmol/L随着浓度增加,促进作用无明显改变,据此确定药物最佳作用浓度为80μmol/L。80μmol/L浓度替普瑞酮处理胃上皮细胞24h后,Transwell实验显示,对照组的迁移率为1.328±0.208,加药组为3.338±0.293,小室膜下蓝染的细胞加药组明显高于对照组(P0.01);划痕实验显示加药组划痕区域细胞迁移率1.00±0.18,对照组0.72±0.08,加药组迁移率明显高于对照组(P0.05)。80μmol/L浓度替普瑞酮处理胃上皮细胞48h后,加药组细胞凋亡率(11.90±1.53)%低于对照组(25.61±0.15)%,差异有统计学意义(P0.01)。结论替普瑞酮能够促进人胃上皮细胞增殖及迁移,抑制人胃上皮细胞凋亡。
[Abstract]:Objective to observe the effects of tipranone on proliferation, migration and apoptosis of normal gastric epithelial cells. Methods MTT assay was used to detect the proliferation of human normal gastric epithelial cells with different concentrations of tipranone and to determine the optimal concentration of tipranone. The effects of the optimal concentration of tipranone on the motility and migration of gastric epithelial cells were detected by scratch test. The effect of optimal concentration of tipranone on apoptosis of gastric epithelial cells was detected by flow cytometry. Results after treated with tipranone for 24 hours, the proliferation of gastric epithelial cells began to appear. From 100.80 渭 mol/L, the effect was more obvious, while 80,320 渭 mol/L had no obvious change with the increase of concentration. The optimal concentration of the drug was 80 渭 mol/L.80 渭 mol/L for 24 h after treatment of gastric epithelial cells. The migration rate of the control group was 1.328 卤0.208, and that of the admixture group was 3.338 卤0.293. The cell migration rate of the subventricular blue staining group was significantly higher than that of the control group P0.01.The scratch test showed that the cell migration rate in the scratch area was 1.00 卤0.18, in the control group was 0.72 卤0.08, and the migration rate in the addition group was significantly higher than that in the control group. The gastric epithelial cells were treated with tipranone at the concentration of P0.05. 80 渭 mol/L for 48 h. The apoptosis rate of the drug added group was lower than that of the control group (11.90 卤1.53% vs 25.61 卤0.15%), and the difference was statistically significant (P 0.01). Conclusion tipranone can promote the proliferation and migration of human gastric epithelial cells and inhibit the apoptosis of human gastric epithelial cells.
【作者单位】：中南大学湘雅医院消化内科;
【基金】：中华消化内镜学会中国胃肠黏膜保护协作组科研基金(D类)资助(201305)
【分类号】：R96

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