生物相容性微乳抗H1N1流感病毒效果及其作用机制的网络分析

发布时间：2018-05-07 06:14

本文选题：生物相容性微乳 + 抗HN流感病毒　；参考：《中华中医药杂志》2017年10期

【摘要】：目的:考察生物相容性微乳(BME)抗H1N1流感病毒的作用,并采用网络药理学方法分析其作用机制。方法:运用四甲基偶氮唑蓝比色法(MTT)检测BME对A549细胞病变抑制率(ER),评价生物相容性微乳的抗病毒效果;利用网络药理学方法构建微乳对H1N1流感作用的网络,拟合微乳抗流感病毒的作用通路,分析其抗H1N1流感病毒的作用机制。结果:BME能够抵抗H1N1流感病毒,其作用呈现一定剂量依赖性,最大抗病毒效果ER为(67.573±2.628)%。与BME抗H1N1流感病毒最相关的4条基因本体相关信号传导通路分别为:MAPK通路、Toll-like受体信号通路、RIG-I-like受体信号通路和Jak-STAT信号通路。结论:BME具有显著抗H1N1流感病毒作用,其作用体现"多成分、多靶点、多通路"的作用特点,BME对H1N1流感的治疗不仅有直接作用,还存在间接的作用关系。
[Abstract]:Aim: to investigate the effect of biocompatible microemulsion (BME) on H1N1 influenza virus and analyze its mechanism by network pharmacology. Methods: the inhibition rate of BME on A549 cells was determined by tetramethyl azolium colorimetry to evaluate the antiviral effect of biocompatible microemulsion, and the network of microemulsion against H1N1 influenza was constructed by network pharmacology. The mechanism of anti-H1N1 influenza virus was analyzed by fitting the anti-influenza virus pathway of microemulsion. Results: BME could resist H1N1 influenza virus in a dose-dependent manner. The maximum antiviral effect was 67.573 卤2.6288.The maximum antiviral effect was 67.573 卤2.6288.The maximum antiviral effect was 67.573 卤2.628%. The four major signal transduction pathways associated with BME against H1N1 influenza virus were: 1 / MAPK / Toll-like receptor signaling pathway, RIG-I-like receptor signaling pathway and Jak-STAT signaling pathway, respectively. ConclusionBME has a remarkable antiviral effect on H1N1, and its action reflects the characteristics of "multi-component, multi-target, multi-pathway" effect. BME has not only direct but also indirect effect on the treatment of H1N1 influenza.
【作者单位】：北京中医药大学;山西中医学院;
【基金】：北京中医药大学研究生自主课题(No.2017-JYB-XS-063) 何首乌两种中药材标准化项目(No.ZYY-2017-154) 国家自然科学基金项目(No.81102815)~~
【分类号】：R96

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