腺相关病毒载体纯化技术的研究进展

发布时间：2018-05-08 20:02

本文选题：腺相关病毒 + 超速离心　；参考：《中国药房》2015年34期

【摘要】：目的:综述腺相关病毒(AAV)载体的分离纯化方法,为AAV的规模化制备提供参考。方法:以"AAV""Purity""Chromatography""腺相关病毒""纯化"等为关键词,组合检索2000年1月-2015年8月在Pub Med、Web of Science、Elsevier、中国知网、维普等数据库中有关AAV分离纯化的文献,选择有代表性的文献进行归纳、总结,分别从AAV纯化的超速离心法、液相色谱法、化学试剂法和超滤法等4个方面进行综述。结果:共查阅到相关文献260余篇,其中有效文献48篇。液相色谱法纯化AAV表现优异,亲和色谱、离子交换色谱等色谱技术已经广泛用于AAV1、2、4、5、6、8、9等不同血清型载体的分离纯化,甚至较难分离的AAV的空壳载体也可以实现分离。但是每种血清型载体的理化性质不尽相同,很难用同一种方法纯化所有载体。结论:液相色谱法在规模化纯化AAV载体具备良好优势,但是应该根据不同血清型载体理化特征建立对应的纯化工艺。
[Abstract]:Aim: to summarize the methods of isolation and purification of adeno-associated virus (AV) vector and to provide reference for the preparation of AAV on a large scale. Methods: using "AAV", "Purity," Chromatography "," adeno-associated virus "and" purification "as the key words, the literature on the separation and purification of AAV from Pub MedAAV Web of Science Elsevier, China knowledge Network, Weip and other databases from January 2000 to August 2015 was searched, and the representative literature was selected to summarize and summarize. In this paper, the purification of AAV by ultracentrifugation, liquid chromatography, chemical reagent and ultrafiltration were reviewed. Results: more than 260 articles were reviewed, 48 of which were effective. The purification of AAV by liquid chromatography is excellent. Affinity chromatography, ion exchange chromatography and other chromatographic techniques have been widely used in the separation and purification of different serotypes, such as AAV1 / AV _ (1), ~ (2 +) ~ (2 +), ~ (-) ~ (5) ~ (-) and so on. Even the empty shell carrier of AAV, which is more difficult to separate, can be separated. However, the physical and chemical properties of each serotype vector are different, so it is difficult to purify all the vectors by the same method. Conclusion: liquid chromatography has a good advantage in the large-scale purification of AAV vectors, but the corresponding purification process should be established according to the physicochemical characteristics of different serotype carriers.
【作者单位】：华侨大学分子药物研究院;九江学院药学与生命科学学院;
【分类号】：R945

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