敞开式质谱成像方法的组织样品稳定性研究

发布时间：2018-05-09 14:22

本文选题：空气动力辅助离子化(AFAI) + 敞开式质谱成像(AMSI)　；参考：《分析测试学报》2017年02期

【摘要】：以添加镇静催眠候选新药N6-羟苄腺嘌呤核苷[N6-(4-hydroxybenzyl)adenine riboside,NHBA]的组织匀浆切片作为考察对象,对空气动力辅助离子化质谱成像系统(Air Flow Assisted Ionization Mass Spectrometry Imaging,AFAI-MSI)的关键参数进行考察与优化,以保证最佳条件进行样品检测。在此基础上,对预处理的整体大鼠组织切片进行平行的连续两次质谱成像分析,考察了其内源性代谢物在成像分析过程中是否发生变化。通过对采集数据中关键质谱峰的筛选等处理步骤,并采用可视化的主成分分析(PCA)方法,开展了组织样品内源性代谢物的稳定性分析,最终验证了采用该样品前处理和质谱成像方法,能够保证组织切片样品中内源性代谢物的稳定性,为质谱成像分析结果提供了可靠依据。
[Abstract]:The key parameters of air Flow Assisted Ionization Mass Spectrometry imaging system (AFAI-MSI) were optimized by using tissue homogenate sections supplemented with N6-(4-hydroxybenzyl)adenine riboside (NHBA), a sedative hypnotic candidate drug. To ensure the best conditions for sample detection. On this basis, the pretreated tissue sections of the whole rat were analyzed by two consecutive mass spectrometry images, and the changes of endogenous metabolites in the process of imaging analysis were investigated. The stability analysis of endogenous metabolites in tissue samples was carried out by screening the key mass spectrum peaks and using the visual principal component analysis (PCA) method. Finally, it is proved that the method of sample pretreatment and mass spectrometry imaging can ensure the stability of endogenous metabolites in tissue slice samples, which provides a reliable basis for the analysis results of mass spectrometry imaging.
【作者单位】：中国医学科学院/北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室;教育部高等教育教学评估中心;中央民族大学生物成像与系统生物学研究中心;
【基金】：国家重大科学仪器设备开发专项资助(2011YQ170067)
【分类号】：R96

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