甲状腺素联合多奈哌齐对成年期甲状腺功能减退症大鼠海马syntaxin-1的影响

发布时间：2018-05-10 05:40

本文选题：甲状腺功能减退症 + 甲状腺素　；参考：《安徽医科大学学报》2015年10期

【摘要】：目的观察甲状腺素(T4)联合多奈哌齐对成年期甲状腺功能减退症(甲减)大鼠的海马内突触前膜相关蛋白1(syntaxin-1)表达的影响,探讨甲减脑损伤及可能的恢复机制。方法 44只SD大鼠随机分为4组:对照组、甲减组、T4替代治疗组及T4和多奈哌齐联合治疗组。通过饮水给予丙基硫氧嘧啶0.05%建立成年期大鼠甲减模型共6周。第5周开始,对照组腹腔注射等量生理盐水,T4替代治疗组每日给予左旋甲状腺素(L-T4)6μg/100 g体重(BW)腹腔注射,联合治疗组每日给予6μg/100g BW L-T4腹腔注射及饮用水中加入0.005%多奈哌齐。采用免疫组织化学法和Western blot法分析4组大鼠海马syntaxin-1的表达情况。结果与对照组比较,甲减组大鼠血清三碘甲状腺原氨酸(T3)、T4水平减低,促甲状腺激素(TSH)水平升高(P0.01),海马内syntaxin-1在CA1、CA3各层及齿状区多形层(DG-PL)表达显著增加(P0.01);T4替代治疗后,血清T3、T4和TSH水平恢复至正常水平,syntaxin-1蛋白的表达未完全恢复到对照组水平(P0.05);T4联合多奈哌齐治疗后syntaxin-1蛋白表达与对照组比较差异无统计学意义。结论 T4联合多奈哌齐治疗可以修复成年期甲减导致的脑损伤。
[Abstract]:Objective to observe the effect of thyroxine T4 (T4) combined with Donepezil on the expression of presynaptic membrane associated protein 1 syntaxin-1 in hippocampus of adult hypothyroidism (hypothyroidism) rats, and to explore the mechanism of hypothyroidism brain injury and recovery. Methods 44 SD rats were randomly divided into 4 groups: control group, hypothyroidism group and T4 replacement therapy group. The adult rat hypothyroidism model was established by drinking 0.05% propylthiouracil for 6 weeks. From the 5th week, the control group was injected intraperitoneally with the same amount of normal saline T4 replacement therapy group, and the control group was given intraperitoneal injection of levothyroxine L-T4 (6 渭 g / 100g BW) daily. The combined treatment group was given 6 渭 g / 100g BW L-T4 intraperitoneal injection daily and 0.005% Donepezil was added to drinking water. The expression of syntaxin-1 in hippocampus of 4 groups was analyzed by immunohistochemical method and Western blot method. Results compared with the control group, the serum levels of triiodothyronine (T3) and thyroid stimulating hormone (TSH) in hypothyroidism group were decreased and the level of TSH was increased (P 0.01). The expression of syntaxin-1 in hippocampus was significantly increased after P0.01T4 replacement therapy in all layers of CA1, CA3 and dentate area. The expression of syntaxin-1 protein in serum T3 T4 and TSH was not completely restored to the control level. There was no significant difference in the expression of syntaxin-1 protein between the control group and the control group after treatment with T4 combined with Donepezil. Conclusion T 4 combined with Donepezil can repair brain injury caused by adult hypothyroidism.
【作者单位】：安徽医科大学附属省立医院老年内分泌科;安徽医科大学第一附属医院老年内分泌科;
【基金】：国家自然科学基金(编号:81272152) 安徽省科技计划项目(编号:12010402134)
【分类号】：R965

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