阿司匹林、维生素A和环磷酰胺对SD大鼠致畸效果的比较研究

发布时间：2018-05-10 23:35

本文选题：阿司匹林 + 维生素A　；参考：《毒理学杂志》2017年03期

【摘要】：目的探讨阿司匹林、维生素A和环磷酰胺对SD大鼠的致畸效果,为致畸试验选择阳性对照物提供参考。方法将SD孕鼠随机分为阿司匹林(AS组,300 mg/kg)、维生素A(VA组,13 mg/kg)、环磷酰胺(CP组,15 mg/kg)和对照组4组,每组25只。AS组和VA组孕鼠于第6~15天灌胃染毒,CP组孕鼠于第12天腹腔注射染毒。孕鼠分别于给受试物后第0、6、9、12、15和20天称重,第20天处死;剖腹后观察活胎数、死胎数、吸收胎数、胎重、胎仔外观和骨骼发育情况。结果阿司匹林、维生素A和环磷酰胺均表现出对SD大鼠的致畸作用,引起胎鼠骨骼畸形率显著增加,差异有统计学意义(P0.05);其中,阿司匹林和环磷酰胺表现出明显的的生殖毒性,与对照组相比,AS组和CP组出现活胎数减少,吸收胎数和死胎数显著增加(P0.05),特别是阿司匹林还导致了母鼠的体重显著降低(P0.05),表现出一定的母体毒性。结论在《食品安全国家标准致畸试验》GB15193.14-2015的推荐剂量下,环磷酰胺在保证足够活胎数的前提下,表现出较为显著的致畸效应,是较为理想的致畸阳性对照物。
[Abstract]:Objective to investigate the teratogenic effects of aspirin, vitamin A and cyclophosphamide on SD rats and to provide reference for teratogenicity test. Methods Sprague-Dawley pregnant rats were randomly divided into three groups: aspirin as group (300 mg / kg), vitamin A VA group (13 mg / kg), cyclophosphamide CP group (15 mg / kg) and control group (n = 25). The pregnant mice were weighed on the 15th and 20th days after the test, and killed on the 20th day. The number of live fetus, the number of dead fetus, the number of absorbed embryos, the fetal weight, the appearance of fetus and the development of bone were observed after laparotomy. Results Aspirin, vitamin A and cyclophosphamide showed teratogenicity in SD rats, which resulted in a significant increase in the rate of skeletal malformation in fetal rats (P 0.05), in which aspirin and cyclophosphamide showed obvious reproductive toxicity. Compared with the control group, the number of live fetus decreased, the number of absorbed embryos and the number of dead fetuses increased significantly in as and CP groups, especially aspirin also resulted in significantly decreased body weight of rats, showing certain maternal toxicity. Conclusion under the recommended dosage of GB15193.14-2015, cyclophosphamide has obvious teratogenic effect on the premise of ensuring enough live fetus, and is an ideal teratogenic positive control.
【作者单位】：上海市疾病预防控制中心;
【基金】：上海市第四轮公共卫生三年行动计划“高端海外研修团队培养计划”(GWTD2015S03)
【分类号】：R99

【参考文献】