香烟烟雾暴露对雄性大鼠血浆尼古

发布时间：2018-05-11 03:04

本文选题：香烟烟雾暴露 + 动物模型　；参考：《中国实验动物学报》2017年06期

【摘要】：目的通过对吸烟大鼠血浆尼古丁、可替宁含量的测定,探讨香烟在体内的内暴露水平。方法清洁级SD雄性大鼠160只,随机分为2、4、6、8、12周的低、中、高染毒剂量组及对照组,每组10只。采用呼吸道静式染毒,每日染毒一次,染毒周期为2、4、6、8、12周。观察大鼠的一般毒性效应,运用气相色谱-质谱联用法检测吸烟大鼠血浆中尼古丁、可替宁的含量。结果香烟烟雾暴露组大鼠在第3周后逐渐开始出现间歇咳嗽,腹式呼吸加剧,鼻噪音等症状,暴露时间越长,症状越明显;染毒剂量越高,染毒周期越长,大鼠体重减少越明显,与空白对照组相比,差异有显著性(P0.01);大鼠血浆尼古丁的保留时间为7.5~8.5 min,随暴露时间的延长,暴露剂量的增加,大鼠血浆中尼古丁含量较空白对照组(155±56.65)ng/m L有所增加;大鼠血浆可替宁的保留时间为11.5~12.5 min;随暴露时间的延长,暴露剂量的增加,大鼠血浆中可替宁含量较空白对照组(340±41.97)ng/m L有所增加,且呈现剂量-反应关系,不同暴露周期间可替宁含量的差异有显著性(P0.05),且暴露时间与暴露剂量存在交互效应(P0.05)。结论香烟烟雾暴露可造成大鼠机体不同程度的损伤,大鼠血浆可替宁浓度能够有效反映香烟烟雾内暴露情况,且呈现较好的剂量-反应关系。
[Abstract]:Objective to study the level of cigarette exposure in vivo by measuring the content of nicotine and cotinine in the plasma of smoking rats. Methods 160 SD male rats of clean grade were randomly divided into low, medium and high dose groups and control groups, with 10 rats in each group. The respiratory tract was infused once a day, and the period of exposure was 12 weeks (2? 4? To observe the general toxicity of rats, the content of nicotine and cotinine in the plasma of smoking rats was determined by gas chromatography-mass spectrometry (GC-MS). Results in the cigarette smoke exposure group, intermittent cough, abdominal breathing, nasal noise and other symptoms began to appear gradually after the third week. The longer the exposure time, the more obvious the symptoms, the higher the dose, the longer the exposure period. Compared with the blank control group, there was a significant difference between the weight loss of rats and the control group, the retention time of plasma nicotine in rats was 7.5 ~ 8.5 min, and the exposure dose increased with the prolongation of exposure time. The content of nicotine in plasma of rats was higher than that of control group (155 卤56.65)ng/m / L), the retention time of cotinine in plasma of rats was 11.5 minutes, and the exposure dose increased with the prolongation of exposure time. The content of cotinine in plasma of rats was significantly higher than that of control group (340 卤41.97)ng/m / L), and showed a dose-response relationship. There was a significant difference in the content of cotinine during different exposure weeks (P 0.05), and there was an interaction between exposure time and exposure dose. Conclusion cigarette smoke exposure can cause different degrees of injury in rats. The plasma levels of ketenin can effectively reflect the exposure to cigarette smoke and show a good dose-response relationship.
【作者单位】：新疆医科大学公共卫生学院;新疆医科大学附属第五医院;
【基金】：国家自然科学基金(No.81260433) 新疆研究生科研创新项目(No.XJGRI2015067) 乌鲁木齐市优秀青年科技人才项目(No.2016SKJRC-2)
【分类号】：R99

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