氧化钕经鼻滴注对小鼠神经系统的影响

发布时间：2018-05-11 06:27

本文选题：氧化钕 + 水迷宫　；参考：《动物医学进展》2017年10期

【摘要】：研究氧化钕经鼻滴注对小鼠神经系统的影响,进一步考察氧化钕的神经毒性是否存在剂量和尺寸效应。选取健康的ICR雌鼠48只,随机分为对照组、低剂量纳米(L-nm)氧化钕组(1μg/mL)、高剂量纳米(H-nm)氧化钕组(40μg/mL)和低剂量微米(L-μm)氧化钕组(1μg/mL)。经鼻染毒,共10d,隔天1次;染毒第2次之后进行水迷宫试验,定位航行试验7d,1d一个单元,同时在第7天进行空间探索试验;取血,进行生化指标检测;取脑置于100g/L甲醛溶液固定,常规脱水,石蜡包埋、切片,苏木素-伊红(HE)染色。剂量效应显示,不同浓度纳米氧化钕对小鼠经鼻滴注,高纳米组与低纳米组相比,目标象限游程占总游程百分比较高(P0.05),但在此尺寸条件下剂量效应不明显。尺寸效应显示,相同浓度纳米氧化钕对小鼠经鼻滴注,L-μm组海马颗粒细胞排列稀疏,细胞脱落;L-nm组海马齿状回颗粒细胞排列较整齐,可见部分细胞核固缩在此试验浓度条件下尺寸效应不明显。血液生物标志物检查试验显示,氧化钕经鼻滴注会对小鼠肝功能、肾功能产生一定的损伤。不同浓度纳米氧化钕经鼻滴注,对小鼠有轻微的刺激学习记忆的作用,但剂量效应不明显。低微米氧化钕对小鼠神经系统有轻微的损伤,但与低纳米氧化钕相比,差异不显著,表明尺寸效应不明显。
[Abstract]:The effects of nasal drip of neodymium oxide on the nervous system of mice were studied and the dose and size effects of the neurotoxicity of neodymium oxide were investigated. 48 healthy female ICR rats were randomly divided into control group, low dose neodymium oxide group (1 渭 g / mL), high dose neodymium oxide group (40 渭 g / mL) and low dose neodymium oxide group (1 渭 g / mL). After the second exposure, water labyrinth test was carried out, a unit of navigation test was located for 7 days, and space exploration test was carried out on the 7th day, blood was taken and biochemical indexes were detected. The brain was fixed in 100g/L formaldehyde solution, dehydrated routinely, embedded in paraffin, sectioned and stained with hematoxylin-eosin. The dose effect showed that the percentage of target quadrant run in the total run was higher in the high nanometer group than in the low nanometer group, but the dose effect was not obvious under the condition of different concentrations of neodymium oxide. The size effect showed that the neodymium oxide at the same concentration was sparsely arranged in hippocampal granulosa cells in the L- 渭 m group, and that in the L-nm group, the granulosa cells in the dentate gyrus of the horse were arranged neatly. It can be seen that the size effect of partial nuclear pyknosis is not obvious at this experimental concentration. Blood biomarker test showed that neodymium oxide could damage liver and kidney function of mice. Different concentrations of nano-neodymium oxide by nasal drip can stimulate learning and memory in mice slightly, but the dose effect is not obvious. Low micron neodymium oxide had slight damage to the nervous system of mice, but the difference was not significant compared with that of low nanometer neodymium oxide, indicating that the size effect was not obvious.
【作者单位】：内蒙古科技大学包头医学院基础医学与法医学院;
【基金】：国家自然科学基金项目(81160341) 内蒙古自治区自然科学基金项目(2015MS0868) 内蒙古自治区研究生科研创新项目[S201610127(Y01)]
【分类号】：R99;TQ133.3

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