基于纳米多孔铂检测细胞中多巴胺

发布时间：2018-05-12 10:02

本文选题：纳米多孔Pt材料 + 多巴胺　；参考：《云南大学学报(自然科学版)》2017年03期

【摘要】：通过还原反应合成纳米多孔Pt材料,并对其进行表征.纳米多孔Pt作为一种纳米模拟酶,对过氧化氢氧化3,3',5,5'-四甲基联苯胺(TMB)形成蓝色氧化产物的反应具有良好的催化作用.加入多巴胺(DA)后,由于DA吸附到纳米多孔Pt的孔道上,减少了催化位点,对纳米多孔Pt的催化作用产生抑制效应,使形成的TMB蓝色氧化产物减少,导致吸光度值下降.研究利用多巴胺对纳米多孔Pt催化作用的抑制效应,采用分光光度计检测体系的吸光度从而实现多巴胺的定量检测.考察了过氧化氢浓度,多孔Pt浓度以及多巴胺反应时间对吸光度的影响.在最优条件下,吸光度值与多巴胺浓度有良好的线性关系,线性范围为0.08~1.0 mmol/L,相关系数为0.995 9,检测下限为0.008 5 mmol/L.该体系为多巴胺的检测提供了一种简单快速的方法,并被用于黑色素瘤细胞裂解液样品中多巴胺的检测.
[Abstract]:Nano-porous Pt materials were synthesized and characterized by reduction reaction. As a kind of nano-mimic enzyme, nano-porous Pt has a good catalytic effect on the oxidation of hydrogen peroxide to the blue oxidation product by the oxidation of 3H _ 2O _ 3, H _ 2O _ 3, H _ 2O _ 5, TMBs, and tetramethyl benzidine (TMBs). After the addition of dopamine (DA), DA adsorbed onto the pore channels of nano-porous Pt, which reduced the catalytic sites and inhibited the catalytic action of nano-porous Pt, resulting in the decrease of the blue oxidation product of TMB and the decrease of the absorbance value. The inhibitory effect of dopamine on the catalytic activity of nano-porous Pt was studied. The absorbance of the system was detected by spectrophotometer to realize the quantitative detection of dopamine. The effects of hydrogen peroxide concentration, porous Pt concentration and dopamine reaction time on absorbance were investigated. Under the optimum conditions, there is a good linear relationship between the absorbance and dopamine concentration. The linear range is 0.081.0 mmol / L, the correlation coefficient is 0.995 9, and the detection limit is 0.008 5 mmol / L. The system provides a simple and rapid method for the detection of dopamine and is used for the detection of dopamine in melanoma cell lysate samples.
【作者单位】：云南师范大学化学化工学院;
【基金】：国家自然科学基金(21465026,21165023)
【分类号】：O657.3;R927

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