瑞巴派特对阿司匹林诱导的小鼠小肠上皮屏障损伤的修复

发布时间：2018-05-13 11:59

本文选题：瑞巴派特 + 阿司匹林　；参考：《中国病理生理杂志》2017年09期

【摘要】：目的:探讨瑞巴派特能否通过促进肠道上皮屏障结构和功能的修复改善阿司匹林导致的小肠黏膜损伤。方法:本研究利用BALB/c小鼠,使用阿司匹林(200 mg·kg~(-1)·d~(-1))连续5 d灌胃的方法制作小肠损伤模型,并根据是否诱导小肠损伤及接受瑞巴派特(320 mg·kg~(-1)·d~(-1))的处理将小鼠分为正常对照组、模型组、瑞巴派特对照组及瑞巴派特治疗组。通过透射电镜、免疫组化、qPCR和Western blot等方法,在不同时点系统地观察瑞巴派特对阿司匹林所致的小鼠小肠黏膜损伤模型中小肠黏膜结构及屏障功能蛋白表达的影响。结果:阿司匹林所致的小肠黏膜损伤小鼠,经瑞巴派特(320 mg·kg~(-1)·d~(-1))连续5 d处理后,小肠上皮细胞间紧密连接结构的损害明显减轻,小肠组织中紧密连接蛋白ZO-1及occludin在mRNA及蛋白质水平的表达均明显增加(P0.05),环氧化酶2(COX-2)和增殖相关信号分子β-catenin、c-Myc的表达也高于对照组(P0.05),组织匀浆中前列腺素E_2浓度显著增加(P0.05),而COX-1的表达在治疗组中无明显改变。同时,治疗组血清中的D-乳酸水平明显降低(P0.05)。结论:瑞巴派特能够通过上调COX-2的表达,促进阿司匹林所致的小鼠小肠黏膜损伤的修复,改善肠屏障的结构与功能。
[Abstract]:Aim: to investigate whether repapet can improve the intestinal mucosal injury induced by aspirin by promoting the repair of intestinal epithelial barrier structure and function. Methods: in this study, BALB/c mice were perfused with aspirin (200 mg / kg) for 5 days to make the model of small intestine injury. The mice were divided into normal control group and model group according to whether to induce small intestine injury and to be treated with repapet (320 mg / kg) DX. Repapet control group and repapet treatment group. By means of transmission electron microscope (TEM), immunohistochemical staining (Western blot) and QPCR, the effects of repapet on the expression of mucosal structure and barrier protein in small and medium-sized intestine of aspirin induced intestinal mucosal injury in mice were systematically observed at different time points. Results: the damage of tight junction between intestinal epithelial cells in aspirin induced intestinal mucosal injury in mice treated with ribavirin 320 mg / kg / d for 5 days was significantly alleviated. The expression of ZO-1 and occludin in small intestine increased significantly in mRNA and protein levels, and the expression of cyclooxygenase 2 (COX-2) and proliferation-related signal molecule 尾 -cateninine c-Myc was also higher than that of control group (P0.05), and the concentration of prostaglandin E _ 2 in tissue homogenate was significantly higher than that in control group (P0.05), and the expression of 尾 -cateninine c-Myc in tissue homogenate was significantly higher than that in control group. The expression of COX-1 did not change in the treatment group. At the same time, the serum D-lactate level in the treatment group was significantly lower than that in the control group (P 0. 05). Conclusion: repapet can improve the structure and function of intestinal barrier by upregulating the expression of COX-2 and promoting the repair of small intestinal mucosal injury induced by aspirin in mice.
【作者单位】：赣州市人民医院消化内科;中山大学孙逸仙纪念医院消化内科;
【基金】：国家自然科学基金资助项目(No.81370475)
【分类号】：R965

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