溶菌酶与EDTA二钠的协同抑菌作用

发布时间：2018-05-15 10:51

本文选题：胞壁质酶 + 依地酸　；参考：《北京大学学报(医学版)》2015年01期

【摘要】：目的:研究溶菌酶与乙二胺四乙酸二钠(ethylenediaminetetraacetic acid disodium salt,EDTA二钠)对粪肠球菌和牙髓卟啉单胞菌的协同抑菌作用。方法:分别培养粪肠球菌和牙髓卟啉单胞菌,并调整菌液浓度至108菌落形成单位(colony-forming unit,CFU)/m L;配制终浓度为0.3、0.5、1、2、5、10、50、100、150和300 g/L的单纯溶菌酶抑菌液,以及添加浓度为0.5、1.0、2.0 g/L的EDTA二钠的混合抑菌液。将菌液与抑菌液作用15 min,加入水溶性四唑盐(water-soluble tetrazolium,WST)工作液染色,酶标仪测定光密度值,计算细菌活性。结果:单纯溶菌酶抑菌液(浓度0.5~150 g/L)对两种细菌的抑菌作用随溶菌酶浓度的升高而增强,且对粪肠球菌的抑菌作用更显著。EDTA二钠与溶菌酶有协同抑菌作用,与溶菌酶浓度相关。溶菌酶浓度为0.5~50 g/L时,EDTA二钠协同溶菌酶作用于粪肠球菌(P0.05),可使其抑菌作用提高约1.2~3.7倍;溶菌酶浓度为0.5~10 g/L时,EDTA二钠协同溶菌酶作用于牙髓卟啉单胞菌(P0.05),可使其抑菌作用提高约1.3~3.5倍;当溶菌酶浓度大于100 g/L时,加入EDTA二钠对细菌均没有明显的协同抑菌作用(P0.05)。结论:对于粪肠球菌及牙髓卟啉单胞菌,在溶菌酶较低浓度时,EDTA二钠有协同抑菌作用;在溶菌酶较高浓度时,EDTA二钠无协同抑菌作用。
[Abstract]:Aim: to study the synergistic bacteriostatic effect of lysozyme and ethylenediaminetetraacetic acid disodium salt EDTA on Enterococcus faecalis and porphyromonas pulposus. Methods: Enterococcus faecalis and porphyromonas pulposus were cultured separately, and the concentration of bacteria solution was adjusted to 108 colony forming unit (CFU / ml). And the mixture of EDTA disodium with concentration of 0.5 ~ 1.0g / L and 2.0 g / L was added. The bacterial solution was treated with bacteriostasis solution for 15 min, and the water soluble tetrazoliumum (WST) working solution was added to stain. The optical density was measured by the enzyme standard instrument, and the bacterial activity was calculated. Results: the bacteriostatic effect of pure lysozyme (0.5 ~ 150g / L) on two bacteria increased with the increase of lysozyme concentration, and the bacteriostatic effect on Enterococcus faecalis was more obvious. EDTA disodium and lysozyme had synergistic bacteriostatic effect, which was related to the concentration of lysozyme. When the concentration of lysozyme was 0.5 g / L, EDTA disodium combined with lysozyme could increase the bacteriostatic activity of Enterococcus faecalis by about 1.2 ~ 3.7 times. When the concentration of lysozyme was 0.5 g / L, EDTA disodium and lysozyme combined with lysozyme could increase the bacteriostatic effect of P0.05G / L, and when the concentration of lysozyme was more than 100g / L, adding EDTA disodium had no obvious synergistic bacteriostatic effect (P0.05N). Conclusion: for Enterococcus faecalis and porphyromonas pulposus, EDTA disodium has synergistic bacteriostatic effect at lower concentration of lysozyme, while EDTA disodium has no synergistic bacteriostatic effect at higher concentration of lysozyme.
【作者单位】：北京大学口腔医学院·口腔医院牙体牙髓科;
【基金】：国家自然科学基金(51103001)资助~~
【分类号】：R96

【参考文献】