小檗碱与克霉唑联用对白念珠菌体外抗菌活性的研究

发布时间：2018-05-15 22:02

  本文选题：小檗碱 + 克霉唑　； 参考：《中国抗生素杂志》2015年05期

【摘要】：目的探讨小檗碱(berberine,BBR)与克霉唑(clotrimazole,CLZ)联合用药时,对白念珠菌(Candida albicans,CA)标准菌株ATCC 10231的体外抑菌效果。方法采用琼脂扩散法(Kirby-Bauer disk diffusion method,KB法)测定BBR、CLZ单用时对CA-10231的最低抑菌浓度(minimum inhibitory concentrations,MIC);把不同浓度BBR与CLZ按棋盘法设计配制加药溶液,采用KB法测定不同浓度BBR与CLZ联用时对CA-10231的MIC;计算部份抑菌浓度指数(fractional inhibitory concentration index,FICI),判断联合抑菌效果,FICI≤0.5为协同作用,0.5FICI≤4为加成作用,FICI4为拮抗作用。结果 BBR和CLZ单用时对CA-10231的平均MIC分别为32000和32μg/m L;不同浓度BBR与CLZ联用时对CA-10231的平均MIC分别为2000μg/m L(P0.01)、8μg/m L(P0.01),平均FICI为0.3125。结论 BBR、CLZ单用时都能抑制CA-10231生长;BBR与CLZ联用时能够以低剂量药物协同抑制CA-10231生长。
[Abstract]:Objective to investigate the bacteriostatic effect of berberine (BBR) combined with clotrimazoleine (clotrimazoleine) on Candida albicans-CA-standard strain ATCC 10231 in vitro. Methods the minimum inhibitory concentration of CA-10231 was determined by Kirby-Bauer disk diffusion method KB method, and the concentration of different concentrations of BBR and CLZ was designed and prepared according to the chessboard method. By using KB method to determine the effect of different concentrations of BBR and CLZ on CA-10231, to calculate the fractional inhibitory concentration index of FICI, and to judge the synergistic effect of FICI 鈮，

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