亚硝酸钠和单硝酸异山梨酯对高肺血流肺动脉高压大鼠肺动脉压力和肺血管重塑的影响

发布时间：2018-05-16 15:00

本文选题：肺动脉高压 + 亚硝酸钠　；参考：《第三军医大学学报》2015年10期

【摘要】：目的比较口服亚硝酸钠和单硝酸异山梨酯对高肺血流肺动脉高压大鼠肺动脉压力和肺血管重塑的影响。方法健康雄性SD大鼠40只,将大鼠按随机数字表法分为假手术组(S组)、肺动脉高压组(P组)、亚硝酸钠组(T1组)、单硝酸异山梨酯组(T2组)。P组、T1组、T2组大鼠通过腹主动脉-下腔静脉分流法建立左向右分流肺动脉高压大鼠模型。饲养11周后连续21 d每天分别给予T1组、T2组大鼠6 mg/kg亚硝酸钠和5 mg/kg单硝酸异山梨酯灌胃干预,余2组给予等量的生理盐水。14周后,监测平均动脉压(MAP)、平均肺动脉压(m PAP)的变化;计算右心室肥厚指数:右心室质量/(左心室质量+室间隔质量)[RV/(LV+S)]、右心室质量/体质量(RV/BW)及(左心室+室间隔质量)/体质量[(LV+S)/BW];硝酸根还原酶法检测血及肺组织中NO含量变化;HE染色光镜下观察肺动脉的病理改变。结果与S组比较,分流手术后P组大鼠m PAP、RV/(LV+S)和RV/BW升高,BW降低,血清NO含量降低(P0.05),光镜下肺动脉管壁增厚,表明肺动脉高压模型建模成功;与P组比较,T1组和T2组m PAP、RV/(LV+S)和RV/BW均明显降低(P0.05),T1组血清NO含量明显升高(P0.05),T2组血清NO含量升高,但差异无统计学意义(P0.05);与T1组比较,T2组m PAP比T1组下降幅度大;T1组、T2组光镜下肺动脉病理学损伤程度明显减轻。4组大鼠MAP、(LV+S)/BW、肺匀浆NO含量比较差异无统计学意义(P0.05)。结论长期口服亚硝酸钠和单硝酸异山梨酯均能降低高肺血流肺动脉高压大鼠平均肺动脉压力,减轻肺血管重塑;单硝酸异山梨酯的降平均肺动脉压力作用大于亚硝酸钠。
[Abstract]:Objective to compare the effects of sodium nitrite and isosorbide mononitrate on pulmonary artery pressure and pulmonary vascular remodeling in rats with pulmonary hypertension. Methods 40 healthy male Sprague-Dawley rats were used. Rats were randomly divided into sham operation group (S group), pulmonary hypertension group (P group), sodium nitrite group (T 1) group and isosorbide mononitrate group (T 2 group). Left to right shunt pulmonary hypertension rat model. After feeding for 11 weeks, rats in T1 group and T2 group were treated with sodium nitrite for 6 mg/kg and isosorbide mononitrate for 5 mg/kg daily for 21 days. The other two groups were given the same amount of normal saline for 14 weeks. The changes of mean arterial pressure (MAPP) and mean pulmonary artery pressure (MPP) were monitored. Calculate right ventricular hypertrophy index: right ventricular mass ratio (left ventricular mass septal mass) [RV/(LV S], right ventricular mass / body mass (RV / BW) and (left ventricular septal mass / body mass [LV S)/BW]; nitrate reductase method for blood and lung tissue detection The changes of no content in the pulmonary artery were observed under light microscope with HE staining. Results compared with group S, after shunt operation, the levels of RV/BW and RV/BW in P group were higher than those in group S, and the serum no content was decreased (P 0.05), and the pulmonary artery wall was thickened under light microscope, which indicated that the model of pulmonary hypertension was successfully established. Compared with the P group, the serum no content in both T 1 group and T 2 group was significantly higher than that in P 0 05 T 1 group, and the serum no content in P 0 05 T 2 group was significantly higher than that in P 0 05 T 2 group, and the serum no content in P 0 05 T 1 group was significantly higher than that in P 0 05 T 2 group. Compared with T1 group, m PAP in T 2 group decreased more than that in T 1 group. The pathological damage of pulmonary artery in T 1 group and T 2 group was significantly alleviated under light microscope. There was no significant difference in no content in lung homogenate compared with that in control group (P 0.05). Conclusion Long-term oral administration of sodium nitrite and isosorbide mononitrate can reduce the mean pulmonary artery pressure and reduce pulmonary vascular remodeling in rats with pulmonary hypertension, and isosorbide mononitrate can decrease the mean pulmonary artery pressure more than sodium nitrite.
【作者单位】：重庆医科大学附属第一医院麻醉科;重庆医科大学附属儿童医院麻醉科;
【基金】：国家自然科学基金(31200853) 卫生部国家临床重点专科建设项目[财社(2011)170号] 重庆市卫生局医学科研计划项目(2012-1-018) 重庆市医学重点学科[渝卫科教(2007)2号]~~
【分类号】：R965

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