肠道菌群对硫酸氢氯吡格雷及其活性代谢产物在大鼠体内药动学的影响

发布时间：2018-05-17 17:00

  本文选题：高效液相色谱-串联质谱 + 硫酸氢氯吡格雷　； 参考：《中国医院药学杂志》2017年21期

【摘要】：目的:探究肠道菌群变化对硫酸氢氯吡格雷及其活性代谢产物在大鼠体内药动学的影响。方法:24只健康大鼠随机分为益生菌组、抗生素组和对照组,每组8只,分别灌胃双歧杆菌乳杆菌三联活菌(0.8 g·kg~(-1))、阿莫西林克拉维酸钾片(125mg·kg~(-1))和等体积的纯化水,连续7 d。第8天给予硫酸氢氯吡格雷片,并于给药前和给药后不同时间点取血于含有衍生试剂的抗凝管中,LC-MS/MS法测定血药浓度,绘制药时曲线,使用DAS 2.1.1拟合药动学参数,SPSS 21.0进行统计学比较。结果:益生菌组、抗生素组和对照组硫酸氢氯吡格雷和活性代谢产物衍生物(CAMD)的主要药动学参数AUC_(0-t)、AUC_(0-∞)、t_(1/2)、t_(max)、CL、V、C_(max)均没有统计学差异(P0.05)。结论:肠道菌群变化对硫酸氢氯吡格雷及其活性代谢产物的药动学参数没有影响。
[Abstract]:Aim: to investigate the effects of intestinal flora changes on the pharmacokinetics of clopidogrel sulfate and its active metabolites in rats. Methods Twenty four healthy rats were randomly divided into three groups: probiotic group, antibiotic group and control group (8 rats in each group). On the 8th day, clopidogrel sulfate tablets were given, and blood samples were collected at different time points before and after administration to determine the concentration of the drug in the anticoagulant tube containing derivative reagent by LC-MS / MS, and to draw the time curve of the drug. The pharmacokinetic parameters of DAS 2.1.1 were statistically compared with that of SPSS 21.0. Results: the main pharmacokinetic parameters of probiotics group, antibiotic group and control group were clopidogrel sulfate and active metabolite derivative CAMD.There was no significant difference in the main pharmacokinetic parameters AUC0 ~ (0)-鈭，

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