阿霉素诱导建立扩张型心肌病大鼠和家兔模型的研究

发布时间：2018-05-20 02:16

本文选题：阿霉素 + 扩心病　；参考：《中药药理与临床》2015年02期

【摘要】：目的:探讨应用阿霉素复制扩张型心肌病大鼠和家兔模型的可行性及方法。方法:取SD大鼠雄性18只,家兔18只,分别给予阿霉素8周,并于给药前、给药后第4、8周利用多普勒超声诊断仪观察给药前后超声心动图变化,HE染色法观察心肌组织病理学的改变。结果:经超声心动图检测,大鼠模型组与对照组比较,左室舒张期末内径(8.9±0.4mm)、左室收缩期末内径(4.6±0.4mm)明显增加、每搏输出量(103.9±13.5mm3)有明显减小(P0.05),家兔模型组与对照组比较,左室舒张期末内径(14.6±0.8mm)、左室收缩期末内径(10.5±0.7mm)明显增加、每搏输出量(665.1±73.6mm3)有明显减小(P0.01),病理学结果证实动物心肌形态变化符合临床心肌病样改变。结论:阿霉素可致实验动物心输出量降低,心肌细胞间质充血,纤维轻度增生,心脏收缩功能降低,与扩张型心肌病临床症状相似,可复制扩张型心肌病模型,为研究其发病机制和有效的治疗方法提供参考依据。
[Abstract]:Objective: to investigate the feasibility and method of using adriamycin to induce dilated cardiomyopathy in rats and rabbits. Methods: eighteen male SD rats and 18 rabbits were given adriamycin for 8 weeks. The changes of echocardiography before and after administration were observed by Doppler echocardiography 8 weeks after administration and the pathological changes of myocardium were observed by HE staining. Results: compared with the control group, the left ventricular end-diastolic diameter was 8.9 卤0.4 mm in the model group and 4.6 卤0.4 mm in the left ventricular systolic end, and the stroke output was 103.9 卤13.5 mm) in the model group, compared with that in the control group. The left ventricular end-diastolic diameter in the model group was significantly lower than that in the control group. The left ventricular end-diastolic diameter (14.6 卤0.8mm) and the left ventricular end-systolic diameter (10.5 卤0.7mm) were significantly increased, and the stroke output (665.1 卤73.6mm) decreased significantly (P 0.01). The pathological results showed that the morphologic changes of the myocardium were consistent with the clinical cardiomyopathy changes. Conclusion: doxorubicin can induce cardiac output, cardiac interstitial congestion, mild hyperplasia of fibers and decrease of cardiac systolic function in experimental animals. It is similar to the clinical symptoms of dilated cardiomyopathy and can be used to replicate dilated cardiomyopathy model. To provide reference for studying the pathogenesis and effective treatment.
【作者单位】：陕西中医学院;陕西中医学院第二附属医院;
【分类号】：R965

【参考文献】