培哚普利对心肌肥厚大鼠中碱性成纤维细胞生长因子的影响

发布时间：2018-05-20 18:34

本文选题：培哚普利 + 心肌肥厚　；参考：《中国临床药理学杂志》2017年18期

【摘要】：目的观察培哚普利对心肌肥厚大鼠传导通路中的碱性成纤维细胞生长因子(b FGF)的作用机制。方法将90只雄性健康SD大鼠随机分为模型组、对照组和实验组,每组各30只。模型组皮下注射异丙肾上腺素3 mg·kg~(-1)·d~(-1)与0.9%Na Cl 2m L灌胃;对照组经皮下注射等剂量0.9%Na Cl与0.9%Na Cl2 m L灌胃;实验组经皮下注射异丙肾上腺素3 mg·kg~(-1)·d~(-1)与培哚普利1 mg·kg~(-1)·d~(-1)溶于0.9%Na Cl 2 m L灌胃。每天1次,共10 d。给药后,测量并计算左心室和心脏的质量指数,观察经苏木精-伊红(HE)染色的左心室心肌组织形态和排列情况,用荧光定量聚合酶链式反应(RT-PCR)检测b FGF mRNA在左心室心肌组织的表达情况。结果实验组、模型组和对照组的左心室质量指数分别为(1.95±0.11),(2.47±0.09),(1.45±0.11)g·m~(-2);心脏质量指数分别为(2.69±0.36),(3.81±0.10),(1.92±0.12)g·m~(-2);心肌细胞中的b FGF mRNA相对表达量分别为0.64±0.11,0.74±0.11,0.38±0.08,实验组分别与对照组和模型组比较,差异均有统计学意义(均P0.05)。结论培哚普利能降低b FGF表达量,从而抑制心室的重构。
[Abstract]:Objective to investigate the mechanism of perindopril on basic fibroblast growth factor (bFGF) in the conduction pathway of myocardial hypertrophy in rats. Methods 90 healthy male Sprague-Dawley rats were randomly divided into model group, control group and experimental group with 30 rats in each group. The model group was given isoproterenol (3 mg 路kg ~ (-1) 路d ~ (-1) and 0.9%Na Cl ~ (2m) L; the control group was given 0.9%Na Cl and 0.9%Na Cl2 mL subcutaneously; the experimental group was subcutaneously injected with isoproterenol 3 mg 路kg ~ (-1) 路d ~ (-1) and perindopril 1 mg 路kg ~ (-1) d ~ (-1). Once a day for 10 days. After administration, the mass index of left ventricle and heart were measured and calculated, and the morphology and arrangement of left ventricular myocardium stained by hematoxylin and eosin were observed. Fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the expression of b FGF mRNA in left ventricular myocardium. Results in the experimental group, the left ventricular mass index in the model group and the control group were 1.95 卤0.111.47 卤0.09 卤1.45 卤0.11g / m ~ (-2), and the cardiac mass index were 2.69 卤0.36 卤0.10 ~ (-1 卤0.10) g / m ~ (-2), respectively. The relative expression of b FGF mRNA in the cardiac myocytes was 0.64 卤0.110.74 卤0.110.38 卤0.08, respectively, compared with the control group and the model group. The difference was statistically significant (P 0.05). Conclusion Perindopril can inhibit ventricular remodeling by reducing the expression of b FGF.
【作者单位】：潍坊市益都中心医院急诊科;潍坊医学院科研处;
【基金】：山东省自然科学基金资助项目(2014ZRB14431)
【分类号】：R965

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