CQMUH-011的抗炎作用研究

发布时间：2018-05-23 09:44

本文选题：CQMUH- + 抗炎作用　；参考：《中国病理生理杂志》2017年04期

【摘要】：目的:观察和探讨CQMUH-011的抗炎作用及其机制。方法:采用二甲苯致小鼠耳肿胀、大鼠棉球肉芽肿和佐剂诱导的大鼠类风湿性关节炎3种模型,观察CQMUH-011对不同炎症模型的抗炎作用,通过耳肿胀度、棉球肉芽肿干重、关节炎指数、足围肿胀度和踝关节病理切片反映相应炎症的严重程度,并利用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测血清中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和白细胞介素6(interleukin-6,IL-6)的水平,相应的试剂盒检测一氧化氮(nitric oxide,NO)、丙二醛(malondialdehyde,MDA)和髓过氧化物酶(myeloperoxidase,MPO)水平。结果:新化合物CQMUH-011能明显降低小鼠耳肿胀度、大鼠棉球肉芽肿干重和踝关节肿胀度,减轻关节炎指数,缓解关节病理损伤;并降低血清中TNF-α、IL-6和NO浓度,减少MDA含量,抑制MPO活性。结论:新化合物CQMUH-011对炎症反应具有明显的抑制作用,这可能与其抑制炎症因子产生和抗脂质过氧化有关。
[Abstract]:Objective: to observe and explore the anti-inflammatory effect of CQMUH-011 and its mechanism. Methods: three models of rat rheumatoid arthritis induced by xylene, cotton ball granuloma and adjuvant were used to observe the anti-inflammatory effects of CQMUH-011 on different inflammatory models. The index of arthritis, swelling degree of foot circumference and pathological section of ankle joint reflect the severity of inflammation. The serum levels of tumor necrosis factor- 伪 (TNF- 伪) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay-ELISA-ELISA. The levels of nitric oxide (no), malondialdehyde (malondialdehyde) (MDAs) and myeloperoxidase (MPO) were detected by the corresponding kit. Results: the new compound CQMUH-011 could significantly reduce ear swelling, dry weight of cotton ball granuloma and swelling degree of ankle joint, alleviate arthritis index, alleviate joint pathological injury, decrease the concentration of TNF- 伪 IL-6 and no in serum, and decrease the content of MDA. Inhibition of MPO activity. Conclusion: the new compound CQMUH-011 has obvious inhibitory effect on inflammatory response, which may be related to its inhibition of inflammatory factor production and anti-lipid peroxidation.
【作者单位】：重庆医科大学重庆市生物化学与分子药理学重点实验室;重庆医科大学药学院药化教研室;
【基金】：重庆市科委基金资助项目(No.cstc2015zdcy-ztzx120003)
【分类号】：R96

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