蛋白酶非水相合成反式头孢丙烯

发布时间：2018-05-25 21:40

  本文选题：非水相 + 蛋白酶　； 参考：《中国抗生素杂志》2015年06期

【摘要】：目的研究非水相体系中反式头孢丙烯的酶法合成工艺条件。方法以7-氨基-3-E-丙烯基-3-头孢菌素-4-羧酸(trans-7-APRA)与对羟基苯甘氨酸(HPG)为底物,利用单甲氧基聚乙二醇修饰化的蛋白酶为催化剂,在非水相体系中合成反式头孢丙烯。采用单因素实验确定影响反式头孢丙烯相对产率的主要因素及其取值范围。以此为基础,应用响应面法对底物配比、酶用量、溶剂配比和温度4因素进行优化。结果非水相体系中反式头孢丙烯均有一定量的合成,以DMSO和甘油的混合非水相体系为最佳合成体系。蛋白酶非水相合成反式头孢丙烯的最佳工艺参数:底物配比ntrans-7-APRA:nHPG=7:10,酶用量HPG:Enzyme=1:1,溶剂比率VDMSO:V甘油=2:1,温度30℃,在此反应条件下相对产率可达到63.6%,与理论预测值相比相对误差在2%左右。结论修饰化中性蛋白酶在非水相体系中可有效合成反式头孢丙烯,该工艺可为酶法制取头孢丙烯的实际应用提供理论依据。
[Abstract]:Objective to study the conditions of enzymatic synthesis of trans cefpropene in non-aqueous phase system. Methods trans-cefpropene was synthesized from 7-amino-3-E- 3-propenyl -3-cephalosporin-4-carboxylic acid trans-7-APRAand p-hydroxyphenylglycine HPGs using monomethoxypolyethylene glycol modified protease as catalyst in the non-aqueous phase system. Single factor experiment was used to determine the main factors affecting the relative yield of trans cefpropene and its range of values. On the basis of this, the four factors of substrate ratio, enzyme dosage, solvent ratio and temperature were optimized by response surface method. Results there was a certain amount of trans-cefpropene synthesis in the non-aqueous phase system, and the mixture of DMSO and glycerol was the best synthesis system. The optimum technological parameters for the synthesis of trans-cefpropene in the non-aqueous phase of protease are as follows: the ratio of substrates ntrans-7-APRA: nHPGN 7: 10, the amount of enzyme used in the synthesis, the ratio of solvent to glycerol is 2: 1, the temperature is 30 鈩，

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