单羧酸转运蛋白1、2、4在急性心衰大鼠模型肾上腺的表达变化

发布时间：2018-05-27 03:13

本文选题：单羧酸转运蛋白、、 + 急性心衰　；参考：《中国临床解剖学杂志》2015年02期

【摘要】：目的观察单羧酸转运蛋白(monocarboxylate transporter,MCT)1、2、4在急性心衰大鼠肾上腺的表达变化,以探讨急性心衰肾上腺的能量代谢变化。方法随机将成年健康SD大鼠(雌雄不限,250±20 g)50只,分为3组:正常组(n=10)、假手术组(n=20,2 subgroups,10 in each)和模型组(n=20,2subgroups,10 in each)。用1.5%戊巴比妥钠静脉注射制作大鼠急性心衰模型;注毕,运用BL-420F生物机能实验系统记录左室内压峰值(LVSP)和左室内压最大上升速率(+dp/dtmax)的变化曲线;用IHC和Western Blot检测模型鼠,肾上腺MCT1,2,4的表达变化。结果在急性心衰发生10 min时,肾上腺皮质和髓质MCT1、2、4的表达均有升高,此时假手术组MCT1、2、4也有升高。与正常对照组相比较,两组的MCT1、2、4的表达升高均具有统计学意义(P0.05);在20 min时,大鼠肾上腺MCT1、2、4的表达较正常组却有所下降,与正常组相比较,仅MCT1的表达下调具有统计学意义(P0.05),而MCT2、4则无明显差别(P0.05)。假手术20 min组与10 min组相比较,MCT1、2、4的表达无明显差别(P0.05)。结论在急性心衰和创伤性应激状态下,肾上腺MCT1、2、4的表达,在十分钟内呈代偿性增加,但随着心衰时间的延长,其表达反而降低,机体的能量代谢出现失代偿,提示MCT1、2、4参与创伤性应激及急性心衰的能量代谢过程。
[Abstract]:Objective to observe the expression of monocarboxylate transporterMCTO1CT-2O4 in adrenal gland of rats with acute heart failure, and to investigate the changes of energy metabolism in the adrenal gland of acute heart failure. Methods Fifty adult healthy SD rats (male and female, 250 卤20 g / L) were randomly divided into 3 groups: normal group (n = 10), sham operation group (n = 20), subgroup (n = 10 in each) and model group (n = 20) (n = 10). The acute heart failure model of rats was made by intravenous injection of 1.5% pentobarbital sodium, and the changes of left ventricular pressure peak pressure (LVSP) and left ventricular pressure maximum rise rate (DP / dtmax) were recorded by BL-420F biological function experiment system after injection, and the model rats were detected by IHC and Western Blot. Changes of expression of MCT1, MCT1 and MCT2 in adrenal gland. Results after 10 min of acute heart failure, the expression of MCT1m2m2m4 in adrenal cortex and medulla was increased, and MCT1m2m4in sham-operated group. Compared with the normal control group, the expression of MCT1m2O4 in both groups was significantly higher than that in the normal control group (P 0.05), but at 20 min, the expression of MCT1m2O4 in the adrenal gland of the rats was lower than that in the normal group, and was significantly lower than that in the normal group. Only the down-regulation of MCT1 expression was statistically significant (P 0.05), but there was no significant difference (P 0.05) between MCT2O4 and MCT2O4. There was no significant difference in the expression of MCT1m2O4 between the 20 min group and the 10 min group in sham operation group (P 0.05). Conclusion under acute heart failure and traumatic stress, the expression of MCT1m2O4 in adrenal gland increases compensatory within 10 minutes, but with the prolongation of heart failure time, the expression of MCT1m2O4 decreases and the energy metabolism of the body becomes decompensated. These results suggest that MCT1 and MCT2 + 4 participate in the energy metabolism of traumatic stress and acute heart failure.
【作者单位】：重庆医科大学神经科学研究中心;
【基金】：重庆医科大学本科生创新实验资助项目(201202) 国家自然科学基金(8100056)
【分类号】：R966

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