四联活菌制剂增加小鼠脾脏和肠系膜淋巴结多种白细胞介素mRNA合成

发布时间：2018-05-27 16:25

本文选题：肠道益生菌 + 小鼠　；参考：《细胞与分子免疫学杂志》2017年09期

【摘要】：目的探讨四联活菌(保加利亚杆菌、幼儿双歧杆菌、芽孢杆菌和嗜酸乳杆菌)制剂对小鼠肠道免疫相关分子的影响。方法将18只BALB/c小鼠随机分成对照组、益生菌培养上清组和菌液组。每只小鼠每天灌胃1次,连续处理1周。实时定量PCR检测脾脏和肠系膜淋巴结组织白细胞介素1α(IL-1α)、IL-2、IL-4、IL-6、IL-12α、IL-17A、IL-17F、IL-10、IL-18和IL-21、α干扰素(IFN-α)、IFN-β、IFN-γ、肿瘤坏死因子α(TNF-α)、转化生长因子β(TGF-β)的m RNA水平,ELISA检测血清IL-17A、IL-21水平。结果与对照组相比,喂食益生菌培养上清组小鼠脾脏组织各种细胞因子的m RNA水平无明显改变,但喂食四联活菌可增加IL-12α、IL-17A、IL-17F和IL-21的m RNA水平;喂食益生菌培养上清和喂食四联活菌均可增加肠系膜组织IL-12α、IL-17F和IL-21的m RNA水平;外周血中IL-17F的水平升高。结论益生菌处理可增加机体白细胞介素水平。
[Abstract]:Objective to investigate the effects of live tetrabacillus (Bulgaria bacillus, Bifidobacterium toddler, Bacillus acidophilus and Lactobacillus acidophilus) on intestinal immune-related molecules in mice. Methods 18 BALB/c mice were randomly divided into control group, probiotic culture supernatant group and bacterial solution group. Each mouse was given intragastric administration once a day for 1 week. Real-time quantitative PCR was used to detect the levels of interleukin-1 伪 -interleukin-1 伪 (IL-2IL-2TNF- 伪) and interleukin-4 (IL-4) IL-6, IL-12 伪, IL-17A, IL-17, IL-10, IL-18, IFN- 伪 and IFN- 尾 IFN- 纬, tumor necrosis factor- 伪 TNF- 伪, transforming growth factor- 尾 (TGF- 尾) in spleen and mesenteric lymph nodes. Elisa was used to detect the level of IL-17AIL-21 in serum. Results compared with the control group, the levels of various cytokines in spleen of mice fed with probiotic culture supernatant did not change significantly, but the levels of IL-12 伪 IL-17, IL-17, IL-17F and m RNA of IL-21 were increased by feeding tetrabacillus. The levels of m RNA of IL-12 伪 IL-17F and IL-21 in mesenteric tissue were increased by feeding probiotic supernatant and tetralogy. The level of IL-17F in peripheral blood was increased. Conclusion Probiotic treatment can increase the level of interleukin.
【作者单位】：南京医科大学附属南京医院;徐州医科大学病理科;
【基金】：国家自然科学基金(81572557) 江苏省青年医学人才项目(QNRC2017077)
【分类号】：R965

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