响应面法优化黑根霉胞外多糖脱蛋白工艺研究

发布时间：2018-05-27 18:51

  本文选题：黑根霉 + 胞外多糖　； 参考：《天然产物研究与开发》2017年10期

【摘要】：采用单因素实验结合Box-Behnken中心组合设计方法,以蛋白质脱除率和综合指数为指标,考察木瓜蛋白酶浓度、酶解温度和酶解时间对黑根霉胞外多糖酶-Sevag法脱蛋白工艺的影响。经过响应面实验优化,确定最优条件为木瓜蛋白酶浓度425 U/mL、酶解温度62℃、酶解时间90 min,经三次重复实验验证,蛋白质脱除率与综合指数均值分别为54.08%和2.07。
[Abstract]:The effects of papain concentration, enzymatic hydrolysis temperature and enzymatic hydrolysis time on the deproteinization of Rhizopus nigra by extracellular polysaccharide enzyme -Sevag method were investigated by single factor experiment and Box-Behnken center combination design. The optimum conditions were determined as follows: papain concentration 425 U / mL, enzymatic hydrolysis temperature 62 鈩，

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