金松双黄酮联用紫杉醇对A549细胞凋亡的影响

发布时间：2018-05-30 09:08

本文选题：紫杉醇 + 金松双黄酮　；参考：《中国临床药理学杂志》2015年19期

【摘要】：目的探讨金松双黄酮联用紫杉醇对A549细胞caspase-3蛋白及Bcl-2、Bax mRNA表达的作用。方法实验分为对照组、紫杉醇组(浓度1μg·L-1)、金松双黄酮+紫杉醇组(浓度分别为0.5,1.0μg·L-1),在药物作用72 h后,用相差显微镜观察A549细胞形态学变化;逆转录-聚合酶链反应检测细胞中Bcl-2、Bax mRNA水平;蛋白免疫印迹法检测caspase-3蛋白的表达。结果与紫杉醇组相比,联用金松双黄酮组较多细胞出现细胞核浓缩、碎裂的凋亡特征,且caspase-3蛋白及Bax mRNA表达上调而Bcl-2mRNA表达下降。结论联用金松双黄酮可增强紫杉醇对A549细胞凋亡作用,可能与调节Bc1-2、Bax及caspase-3的表达水平有关。
[Abstract]:Objective to investigate the effects of Jin Song diflavonoids combined with paclitaxel on the expression of caspase-3 protein and Bcl-2P Bax mRNA in A549 cells. Methods the experiment was divided into two groups: paclitaxel group (1 渭 g / L) and Jin Song dicoflavone paclitaxel group (0.5 渭 g / L ~ (-1) respectively. After 72 h of treatment, the morphological changes of A549 cells were observed by phase contrast microscope. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the level of Bcl-2P Bax mRNA, and Western blot was used to detect the expression of caspase-3 protein. Results compared with paclitaxel group, the apoptotic characteristics of cells in combination with Jin Song bisflavone group were nuclear concentration and fragmentation, and the expression of caspase-3 protein and Bax mRNA were up-regulated and the expression of Bcl-2mRNA was lower than that of paclitaxel group. Conclusion the effect of paclitaxel on apoptosis of A549 cells can be enhanced by the combination of Jin Song bisflavone, which may be related to the regulation of the expression of Bc1-2Tbx and caspase-3 in A549 cells.
【作者单位】：齐齐哈尔医学院药理教研室;齐齐哈尔医学院药学院实验中心;齐齐哈尔医学院病原教研室;齐齐哈尔医学院中药教研室;
【基金】：黑龙江省教育厅科学技术研究基金资助项目(12531834)
【分类号】：R96

【共引文献】