口服富氢水对小鼠脂肪肝缺血再灌注损伤的保护作用

发布时间：2018-05-30 18:52

  本文选题：富氢水 + 非酒精性脂肪肝　； 参考：《山东大学学报(医学版)》2015年01期

【摘要】：目的探讨富氢水对脂肪肝缺血再灌注损伤小鼠模型的保护作用。方法将30只C57BL/6小鼠随机分为3组。假手术组:喂蛋氨酸胆碱缺乏高脂饮食非酒精性脂肪肝造模;对照组:肝脏缺血再灌注前灌服蒸馏水;富氢水缺血再灌注组:肝脏缺血再灌注前灌服富氢水,再灌注6 h后处死小鼠,检测血清中AST和ALT含量,观察肝脏组织形态学变化,分析肝脏组织损伤程度、巨噬细胞浸润、炎性因子TNF-α、IL-6、OPN mRNA表达水平和肝脏组织内活化的Caspase-3蛋白表达水平。结果与对照组相比较,富氢水组血清ALT和AST水平较低(P0.05),巨噬细胞浸润和凋亡细胞明显减少,炎性因子mRNA的表达明显降低。Caspase-3表达明显降低。结论富氢水能够减轻脂肪肝小鼠肝脏缺血再灌注损伤,其机制与富氢水抑制氧化应激反应和炎症反应,进而抑制Caspase-3蛋白表达有关。
[Abstract]:Objective to investigate the protective effect of hydrogen rich water on fatty liver ischemia reperfusion injury in mice. Methods 30 C57BL/6 mice were randomly divided into 3 groups. Sham-operation group: model of non-alcoholic fatty liver induced by methionine choline deficiency diet; control group: distilled water before hepatic ischemia-reperfusion; Hydrogen-rich water ischemia-reperfusion group: before liver ischemia-reperfusion, After reperfusion for 6 h, the mice were killed, the contents of AST and ALT in serum were measured, the changes of liver histomorphology were observed, the degree of liver injury and the infiltration of macrophages were analyzed. The expression level of inflammatory factor TNF- 伪 and IL-6 mRNA and the expression of activated Caspase-3 protein in liver tissue. Results compared with the control group, the levels of serum ALT and AST were lower, macrophage infiltration and apoptotic cells were significantly decreased, and the expression of inflammatory factor mRNA was significantly decreased in the hydrogen-rich water group than that in the control group. Conclusion Hydrogen-rich water can attenuate hepatic ischemia-reperfusion injury in fatty liver mice, and its mechanism is related to the inhibition of oxidative stress and inflammation by hydrogen-rich water, and thus the inhibition of Caspase-3 protein expression.
【作者单位】： 内蒙古农业大学生命科学学院;内蒙古自治区生物制造重点实验室;
【基金】：内蒙古自然科学基金重大项目(2010ZD10)
【分类号】：R965
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本文编号：1956397