瑞替加滨对离体大鼠十二指肠平滑肌收缩功能的影响及其机制

发布时间：2018-05-31 00:06

本文选题：瑞替加滨 + 十二指肠　；参考：《山东医药》2017年20期

【摘要】：目的探讨瑞替加滨对离体大鼠十二指肠平滑肌收缩功能的影响及其机制。方法取大鼠十二指肠肠管剪成长度约1.5 cm的肠段,采用BL-420F生物机能实验系统持续记录张力曲线。十二指肠肠管张力曲线稳定后行以下试验:(1)依次予3.125、6.25、12.5、25、50、100、150、200μmol/L的瑞替加滨溶液干预,每个浓度作用时间均为15 min,中间用Kreb's溶液冲洗20 min。测量瑞替加滨干预前及各浓度干预后十二指肠肠管平滑肌张力曲线的平均振幅。(2)加入10μmol/L XE-991作用15 min,再加入20μmol/L瑞替加滨作用15 min。计算瑞替加滨加药前后离体十二指肠肠管平滑肌张力曲线的平均振幅变化百分率。(3)加入20μmol/L瑞替加滨作用15 min,再加入10μmol/L XE-991作用15 min。比较XE-991加药前后离体十二指肠肠管平滑肌张力曲线的平均振幅变化百分率。结果 (1)瑞替加滨作用下离体十二指肠肠管平滑肌张力曲线的平均振幅(基线值)变化百分率为100%。各浓度瑞替加滨作用下张力曲线的平均振幅变化百分率均低于基线值,P均0.05。在6.25~200μmol/L范围内,随着瑞替加滨浓度增加提高,平均振幅变化百分率逐渐下降,P均0.05。(2)瑞替加滨加药前后XE-991孵育的离体十二指肠肠管平滑肌张力曲线的平均振幅变化百分率无统计学差异,P0.05。(3)XE-991加药后瑞替滨孵育的离体十二指肠肠管平滑肌张力曲线的平均振幅高于加药前,P0.05。结论瑞替加滨在6.25~200μmol/L范围内可呈剂量依赖性抑制大鼠离体十二指肠平滑肌收缩;其机制可能为激活钾离子通道。
[Abstract]:Objective to investigate the effect and mechanism of retegabine on contractile function of isolated rat duodenal smooth muscle. Methods the tension curves were recorded continuously by BL-420F biological function experiment system in the intestinal segment of rat duodenum which had a growing degree of 1.5 cm. After the tension curve of duodenum was stabilized, the following experiment: 1: 1) was treated with retegabine solution of 3.125 ~ 6.25 ~ 12.5 ~ (12.5) ~ 2550 ~ 100 ~ 150200 渭 mol/L. The time of treatment for each concentration was 15 minutes, and the Kreb's solution was used to wash the solution for 20 minutes. The mean amplitude of tension curve of duodenal smooth muscle was measured before and after the intervention of retegabine. The mean amplitude of tension curve of duodenal smooth muscle was measured. 10 渭 mol/L XE-991 was added for 15 minutes, and then 20 渭 mol/L retegabine was added for 15 minutes. The mean amplitude change percentage of tension curve of isolated duodenal smooth muscle was calculated before and after retegabine was added. (3) 20 渭 mol/L retegabine was added for 15 minutes, and then 10 渭 mol/L XE-991 was added for 15 minutes. To compare the average amplitude percentage of tension curve of isolated duodenal smooth muscle before and after administration of XE-991. Results 1) the percentage of mean amplitude (baseline value) of tension curve of isolated duodenal smooth muscle under retegabine was 100%. The percentage of mean amplitude change of tension curve under the action of retegabine was lower than that of baseline value (P < 0.05). In the range of 6.25 渭 mol/L, with the increase of retegabine concentration, The percentage of mean amplitude change decreased gradually (P < 0.05. 2) the average amplitude change percentage of tension curve of isolated duodenal smooth muscle incubated with XE-991 before and after retegabine administration had no statistical difference. The mean amplitude of smooth muscle tension curve of somatoduodenum was higher than that of P0.05 before administration. Conclusion Retigabine can inhibit the contraction of isolated rat duodenal smooth muscle in a dose-dependent manner in the range of 6.25 渭 mol/L, and the mechanism may be the activation of potassium channel.
【作者单位】：中国医科大学药学院;
【基金】：国家自然科学基金资助项目(81270900,81670492)
【分类号】：R965

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