基于微电极阵列的大鼠脑内L-DOPA刺激谷氨酸释放和神经电信号检测

发布时间：2018-05-31 06:58

  本文选题：微机电系统 + 植入式微电极阵列　； 参考：《纳米技术与精密工程》2017年06期

【摘要】：左旋多巴(L-DOPA)是帕金森症的主要治疗药物之一,长期使用具有神经毒性,其作用机理复杂.为了研究L-DOPA对大脑纹状体谷氨酸神经通路在体神经元的影响,采用微机电系统(MEMS)技术制备了一种16通道植入式微电极阵列(MEA)芯片,其上集成了直径10μm的电生理、电化学双模信号检测位点,能够实时记录脑内神经递质谷氨酸和动作电位(spike)、局部场电位(LFP)等神经电信号的同步变化.将这种MEA植入大鼠纹状体,在皮层施加L-DOPA(100μL,2 mmol/L)刺激后,随着纹状体内谷氨酸浓度的迅速增加,神经元spike发放频率和LFP低频段功率同步衰减.实验结果从神经元层次在体验证了L-DOPA的神经毒性,可为帕金森症治疗药理研究提供线索.
[Abstract]:L-DOPA is one of the main drugs for Parkinson's disease. It has neurotoxicity and complex mechanism. In order to study the effect of L-DOPA on the in vivo neurons of the glutamate pathway in the striatum of the brain, a 16 channel implanted microelectrode array (MEA) chip was fabricated by using the microelectromechanical system (MEMS) technique. The microelectrode array was integrated with electrophysiology of 10 渭 m in diameter. The detection site of electrochemical double mode signal can record the synchronous changes of neurotransmitter glutamic acid and action potential spike, local field potential (LFPP) and so on in the brain in real time. The MEA was implanted into the striatum of rats and stimulated by L-DOPA(100 渭 L ~ (2 mmol / L) in cortex. With the rapid increase of glutamate concentration in the striatum, the frequency of neuronal spike emission and the power of LFP decreased synchronously. The results confirm the neurotoxicity of L-DOPA in vivo and provide clues for the pharmacological study of Parkinson's disease.
【作者单位】： 中国科学院电子学研究所传感技术联合国家重点实验室;中国科学院大学;
【基金】：国家自然科学基金资助项目(61527815,31500800,61501426,61471342) 国家重点研发计划资助项目(2017YFA0205900) 北京市科技计划资助项目(Z141100000214002,Z141102003414014) 中国科学院重点资助项目(KJZD-EW-L11-2)
【分类号】：R96
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本文编号：1958728