牛磺酸对尼古丁致大鼠睾丸氧化损伤的保护作用

发布时间：2018-06-01 07:58

本文选题：牛磺酸 + 尼古丁　；参考：《环境与职业医学》2015年12期

【摘要】：[目的]探讨牛磺酸对尼古丁所致雄性大鼠睾丸氧化损伤的保护作用。[方法]32只SD雄性大鼠,随机分为阴性对照组、牛磺酸对照组、尼古丁染毒组及牛磺酸干预组。阴性对照组大鼠生理盐水1 m L皮下注射,双蒸水1 m L灌胃;牛磺酸对照组大鼠皮下注射生理盐水1 m L,灌胃牛磺酸双蒸水溶液1 m L(牛磺酸量为100 mg/kg);尼古丁染毒组大鼠皮下注射尼古丁生理盐水溶液1 m L(尼古丁量为2 mg/kg),灌胃双蒸水1 m L;牛磺酸干预组大鼠如上注射尼古丁生理盐水溶液和灌胃牛磺酸双蒸水溶液。皮下注射和灌胃每日1次,连续4周。测定大鼠睾丸系数、附睾系数、精子密度和活率;检测睾丸组织丙二醛(MDA)含量;检测睾丸组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性。[结果]睾丸系数、附睾系数、精子密度和活率:尼古丁染毒组大鼠显著低于另3组(P0.05),牛磺酸干预组显著低于阴性对照组和牛磺酸对照组(P0.05);睾丸组织MDA含量:尼古丁染毒组大鼠显著高于另3组(P0.05),牛磺酸干预组显著高于阴性对照组和牛磺酸对照组(P0.05);睾丸组织SOD、CAT、GSH-Px活性:尼古丁染毒组大鼠显著低于另3组(P0.05),牛磺酸干预组显著低于阴性对照组和牛磺酸对照组(P0.05)。[结论]尼古丁可致大鼠睾丸组织氧化损伤,牛磺酸对此具有抗氧化保护作用。
[Abstract]:Objective to investigate the protective effect of taurine on oxidative injury of testis induced by nicotine in male rats. [methods] Thirty-two male SD rats were randomly divided into negative control group, taurine control group, nicotine exposure group and taurine intervention group. Rats in the negative control group were subcutaneously injected with 1 mL normal saline, and 1 mL double steamed water was administered intragastrically. Rats in the taurine control group were subcutaneously injected with 1 mL of normal saline and were given 1 mL of taurine double-steamed aqueous solution (taurine 100 mg / kg 路kg ~ (-1), while those in the nicotine group were subcutaneously injected with 1 mL of nicotine (2 mg / kg) of nicotine. The rats in the taurine intervention group were given nicotine saline solution and taurine double distilled water solution. Subcutaneous injection and gavage were given once a day for 4 weeks. The testicular coefficient, epididymal coefficient, sperm density and viability, the content of malondialdehyde (MDA) in testis, the activities of superoxide dismutase (SOD), catalase (catalase) and glutathione peroxidase (GSH-Px) in testis were measured. [results] testicular coefficient, epididymal coefficient, Sperm density and motility were significantly lower in the nicotine exposure group than in the other three groups, and significantly lower in the taurine intervention group than in the negative control group and the taurine control group, and the MDA content in testicular tissue was significantly higher in the nicotine exposure group than in the other three groups. The activity of GSH-Px in testicular tissue of rats exposed to nicotine was significantly lower than that of the other three groups (P 0.05), and that of taurine intervention group was significantly lower than that of negative control group and taurine control group (P 0.05), and the activity of GSH-Px in testicular tissue was significantly lower in the nicotine exposed group than that in the other three groups (P 0.05), and that in the taurine intervention group was significantly lower than that in the negative control group and the taurine control group. [conclusion] nicotine can induce oxidative injury of testis in rats, taurine has antioxidant protective effect on it.
【作者单位】：解放军第210医院妇产科;解放军第210医院病理科;解放军第210医院医务处;大连大学附属中山医院妇产科;北京军区总医院妇产科;
【分类号】：R965

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