乙酸诱导建立大鼠急性直肠黏膜损伤模型的形态学观察

发布时间：2018-06-01 15:42

本文选题：急性直肠黏膜损伤 + 大鼠模型　；参考：《中国实验动物学报》2015年02期

【摘要】：目的通过形态学观察评价乙酸诱导建立急性直肠黏膜损伤模型的效果。方法将含有4%乙酸溶液的棉签经SD大鼠肛门置入直肠1 min,置入深度为3 cm,诱导大鼠形成急性直肠黏膜损伤,并于损伤后0.5 h和1、4、6 d分别解剖大鼠,对直肠进行大体形态观察和组织病理学分析。结果损伤后大鼠6 d内全部存活,模型成功率为100%。损伤后0.5 h~1 d,大鼠直肠黏膜大体形态由充血、水肿和溃疡到合并出血,镜下形态见黏膜层由上皮组织坏死伴出血到黏膜组织全层坏死,腺体由部分留存到全无,黏膜下层由水肿到合并充血、出血,间质内由血管扩张充血到炎细胞浸润;损伤后4~6 d,直肠黏膜大体形态仍可见部分水肿和血丝,镜下形态见黏膜上皮有部分留存,损伤部位有炎性肉芽组织增生,黏膜充血、出血减轻。结论 4%乙酸作用于直肠1 min能成功诱导建立大鼠急性直肠黏膜损伤模型,该模型操作方便、成功率高、重复性好,损伤维持时间6 d以上,适合作为快速筛选直肠外用药物疗效的动物模型。
[Abstract]:Objective to evaluate the effect of acute rectal mucosal injury induced by acetic acid by morphological observation. Methods the cotton swabs containing 4% acetic acid solution were implanted into the rectum of SD rats for 1 min with a depth of 3 cm. The rats were induced to form acute rectal mucosal injury. The rats were dissected at 0.5 h and 1 day 4 and 6 days after the injury. Gross morphology and histopathology of rectum were observed. Results all the rats survived within 6 days after injury, and the success rate of the model was 100%. At 0. 5 h after injury, the morphology of rectal mucosa in rats ranged from hyperemia, edema and ulceration to combined bleeding. Under microscope, the mucosal layer was found to be from necrosis of epithelial tissue with hemorrhage to necrosis of whole layer of mucosal tissue, and gland was retained from part to none. The submucosa from edema to hyperemia, hemorrhage, interstitial vasodilation to inflammatory cell infiltration, 4 days after injury, rectal mucosal gross morphology can still be seen in some edema and blood filaments, microscopic morphology of mucosal epithelium is partially retained. Injury site has inflammatory granulation tissue proliferation, mucosal congestion, bleeding relief. Conclusion the rat model of acute rectal mucosal injury induced by 4% acetic acid for 1 min can be successfully induced. The model has the advantages of convenient operation, high success rate, good reproducibility, and lasting for more than 6 days. It is suitable as an animal model for rapid screening of the efficacy of external rectal drugs.
【作者单位】：广东省医学实验动物中心比较医学实验室;广东省佛山市南海区第三人民医院;
【基金】：国家自然科学基金项目(No.81403212) 广东省医学科研基金(No.B2014062) 广东省中医药管理局基金(No.20142019)
【分类号】：R-332;R965

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