苯磺酸左旋氨氯地平对兔内皮损伤术后内膜增生与斑块稳定的影响

发布时间：2018-06-03 18:25

本文选题：苯磺酸左旋氨氯地平 + 瑞舒伐他汀　；参考：《临床心血管病杂志》2015年01期

【摘要】：目的:观察苯磺酸左旋氨氯地平对新西兰兔腹主动脉球囊内皮损伤术后动脉粥样硬化(AS)的影响,从抗炎和稳定斑块的角度探讨相关机制。方法:42只新西兰兔随机分为假手术组、模型组、左旋氨氯地平组以及瑞舒伐他汀组。模型采用高脂饮食联合腹主动脉球囊内皮损伤术制作。ELISA法检测各组术前与术后8周的血脂、血管炎症性及斑块稳定性相关指标,如超敏C反应蛋白(hs-CRP)、脂蛋白磷脂酶A2(Lp-PLA2)以及1型纤溶酶原激活物抑制物(PAI-1),术后8周处死后取腹主动脉病变组织,行HE染色分析斑块厚度及内膜增生系数(IHI)以及免疫组织化学法检测斑块内金属基质蛋白酶(MMP-3)表达。结果:左旋氨氯地平组血清低密度脂蛋白胆固醇(LDL-C)水平较模型组有降低趋势(16.24mmol/L∶19.41mmol/L,P=0.08)。左旋氨氯地平组的腹主动脉斑块厚度、IHI与模型组无显著性差异(313μm∶405μm、0.68∶0.66,均P0.05),而瑞舒伐他汀组的斑块厚度、IHI明显低于模型组(273.75μm∶405.93μm、0.52∶0.68,均P0.05)。左旋氨氯地平组的血清hs-CRP、Lp-PLA2以及PAI-1水平与模型组无明显差异(P0.05),而左旋氨氯地平组斑块内MMP-3阳性表达面积百分比显著小于模型组(52.45%∶70.84%,P0.05)。结论:左旋氨氯地平具有降低血清LDL-C趋势,对抑制兔腹主动脉球囊内皮损伤术后的内膜增生无明显作用,但可通过抑制斑块内MMP-3的表达从而增加斑块稳定性。
[Abstract]:Aim: to observe the effect of levamlodipine on atherosclerosis (ASS) after balloon endothelial injury of abdominal aorta in New Zealand rabbits and to explore the mechanism of anti-inflammatory and stable plaque. Methods 42 New Zealand rabbits were randomly divided into sham operation group, model group, levamlodipine group and resuvastatin group. The serum lipids, vascular inflammation and plaque stability were measured by high fat diet combined with balloon endothelial injury of abdominal aorta by Elisa before and 8 weeks after operation. For example, hypersensitive C-reactive protein (hs-CRPN), lipoprotein phospholipase A2P (Lp-PLA2) and plasminogen activator inhibitor type 1 (PAI-1) were taken from abdominal aorta after 8 weeks of operation. The thickness of plaque, intimal hyperplasia coefficient (IHI) and the expression of metalloproteinase 3 (MMP-3) in plaque were detected by HE staining and immunohistochemistry. Results: compared with the model group, the serum LDL-C level of the L-amlodipine group decreased to 16.24 mmol / L ~ (19.41) mmol / L ~ (0.08) mg 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1). There was no significant difference in the thickness of abdominal aortic plaque (IHI) between the levamlodipine group and the model group. There was no significant difference between the model group and the model group (313 渭 m: 405 渭 m or 0.68: 0.66, P < 0.05), while the thickness of the plaque in the rosuvastatin group was significantly lower than that in the model group (273.75 渭 m vs 405.93 渭 m: 0.520.68, P 0.05 5). There was no significant difference in the levels of hs-CRPnLp-PLA2 and PAI-1 between the L-amlodipine group and the model group, but the percentage of MMP-3 positive area in the L-amlodipine group was significantly lower than that in the model group (52.45: 70.84). Conclusion: L-amlodipine can decrease the trend of serum LDL-C and has no effect on intimal hyperplasia after abdominal aortic balloon endothelial injury in rabbits, but it can increase plaque stability by inhibiting the expression of MMP-3 in plaque.
【作者单位】：暨南大学附属第一医院心内科;
【基金】：广东省科技计划项目(No:2011B031800336)
【分类号】：R965

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