乌司他丁联合去氧氟尿苷对乳腺癌肿瘤微环境的影响

发布时间：2018-06-06 09:39

本文选题：乳腺癌 + 调节性T细胞　；参考：《中国新药杂志》2017年14期

【摘要】：目的:探讨乌司他丁(ulinastatin,UTI)联合去氧氟尿苷(doxifluridine,DFD)对肿瘤生长及肿瘤微环境的影响。方法:构建Balb/c小鼠荷4TI乳腺癌肿瘤模型,将70只小鼠随机分为空白组,2个单药组以及4个UTI合用DFD组共7组分别给药,计算肿瘤抑制率;流式细胞术检测Treg细胞,各功能树突状细胞;Western blot检测IL-10及TGF-β表达;免疫组化法检测CD34表达及肿瘤细胞凋亡情况。结果:对比空白组,各用药组均能抑制肿瘤生长,Treg/CD4~+T细胞的比值(P0.001),IL-10与TGF-β的表达量(P0.001)及CD34分子的表达(P0.001)均降低,各功能树突状细胞(P0.001)和肿瘤细胞的凋亡(P0.001)增加,UTI合用3 mg DFD组实验效果最显著。结论:UTI联合DFD可抑制免疫正常小鼠乳腺癌的生长,各组中以UTI联合3 mg DFD效果最为明显。其机制可能与调节Treg细胞以及DC细胞抗原提呈相关因子有关。
[Abstract]:Aim: to investigate the effects of ulinastatin (UTI) combined with dofluridine (DFDD) on tumor growth and tumor microenvironment. Methods: the tumor model of 4TI breast cancer was established in Balb/c mice. 70 mice were randomly divided into blank group, single drug group and 4 UTI combined with DFD groups. The tumor inhibition rate was calculated by flow cytometry, and Treg cells were detected by flow cytometry. The expression of IL-10 and TGF- 尾 in dendritic cells was detected by Western blot, and the expression of CD34 and apoptosis of tumor cells were detected by immunohistochemistry. Results: compared with the blank group, the ratio of Treg-CD4T cells to TGF-IL-10 and TGF- 尾 and the expression of CD34 molecule (P0.001) and the expression of TGF- 尾 (P0.001) were decreased in each treatment group. P0.001) and apoptosis of tumor cells (P0.001) increased significantly in combination with 3 mg DFD. Conclusion DFD combined with DFD can inhibit the growth of breast cancer in immunized normal mice. The effect of UTI combined with 3 mg DFD is the most obvious in each group. The mechanism may be related to the regulation of antigen presentation in Treg cells and DC cells.
【作者单位】：重庆医科大学附属第二医院三腺外科;
【基金】：重庆市科委社会事业与民生保障科技创新专项一般项目(cstc2016shmszx130012)
【分类号】：R96

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