还原型谷胱甘肽对肾急性缺血再灌注性损伤的作用

发布时间：2018-06-15 18:03

本文选题：还原型谷胱甘肽 + 预处理　；参考：《实用医学杂志》2016年08期

【摘要】：目的:探讨还原型谷胱甘肽(GSH)预处理及后处理对大鼠肾脏急性缺血再灌注性损伤(IRI)的作用。方法:50只SD成年大鼠分成5组:假手术对照组(Sham组),缺血再灌注组(I/R组),GSH预处理组(Pre组),GSH后处理组(Post组),每组10只。Pre组在术前24、16、8 h和45 min时腹腔注射4%GSH 100mg/kg。Post在术后45 min、6、12和18 h腹腔注射同等剂量的GSH。各组动物制备缺血再灌注模型后第24小时检测血清肌酐(Cr),尿素氮(BUN)水平,总超氧化物歧化酶(T-SOD)活性,丙二醛(MDA)和一氧化氮(NO)含量。HE染色观察组织病理学改变。结果:与I/R组相比,Pre组肾组织结构损害明显减轻,Post组无明显病理改变,Pre组和Post组血清Cr、BUN、MDA和NO含量减少(P0.05),T-SOD含量增加(P0.05),Post组T-SOD含量高于Pre组(P0.05)。结论:肾IR前或后24 h内使用GSH对肾脏均有保护作用,IR后使用GSH对肾脏的保护作用更优。
[Abstract]:Aim: to investigate the effects of reduced glutathione (GSH) preconditioning and postconditioning on acute renal ischemia reperfusion injury (IRI) in rats. Methods Fifty adult Sprague-Dawley rats were divided into five groups: sham-operated control group (Sham group), ischemia reperfusion group (I / R group), preconditioning group (pre group) and post treatment group (10 rats in each group). 10 rats in each group were injected intraperitoneally with 4GSH 100mg / kg 路Post after post operation 24: 16 h and 45 min before operation. The same dose of GSH was injected intraperitoneally for 12 and 18 hours at 45 min. The serum creatinine (Cr), urea nitrogen (bun), total superoxide dismutase (T-SOD) activity, malondialdehyde (MDA) and nitric oxide (no) contents were detected 24 hours after ischemia reperfusion in each group. The histopathological changes were observed by HE staining. Results: compared with the I / R group, the renal tissue structure damage in the pre group was significantly reduced. The contents of MDA and no in the serum of pre group and Post group were significantly reduced compared with that in the I / R group. The content of T-SOD in the P0.05 group was significantly higher than that in the pre group (P0.05%). The content of T-SOD in the post group was higher than that in the pre group. Conclusion: GSH has protective effect on kidney before or within 24 hours after IR. The protective effect of GSH on kidney is better after IR.
【作者单位】：西南医科大学肿瘤研究所;西南医科大学第一附属医院儿科;西南医科大学第一附属医院肾病内科;
【基金】：泸州医学院人才基金资助项目(编号:11192)
【分类号】：R965

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