他达拉非延缓大鼠慢性移植血管硬化病变

发布时间：2018-06-16 18:49

  本文选题：他达拉非 + 移植物闭塞　； 参考：《实用医学杂志》2015年11期

【摘要】：目的:探讨他达拉非对大鼠慢性移植动脉血管病变保护作用。方法:实验分3组:同系移植组,供、受者为Lewis大鼠,无处理;异系移植实验组,Lewis大鼠接受Brown-Norway(BN)大鼠腹主动脉移植;干预组他达拉非0.5 mg/(kg·d),Lewis大鼠接受BN大鼠腹主动脉移植。术后4周和8周取移植动脉,组织学观察、测量内膜和中膜厚度,Western blot检测PKG-Ⅰ、Rho A蛋白表达。收集各组血浆,ELISA检测c GMP浓度。结果:异系移植实验组移植动脉呈移植物血管病表现,血管内膜显著增厚;干预组血管内膜增厚,与实验组相比显著减轻(P0.05);干预组血浆c GMP浓度较实验组显著增高(P0.05);与实验组相比,干预组PKG-Ⅰ显著增高、Rho A明显降低(P0.05)。结论他达拉非通过调控c GMP/PKG-Ⅰ和Rho A通路,抑制移植物血管病发展。
[Abstract]:Objective: to investigate the protective effect of tadarafil on chronic graft artery disease in rats. Methods: the experiment was divided into three groups: the same transplantation group, the recipient group was Lewis rats without treatment, the experimental group of allogeneic Lewis rats received Brown-NorwayBN rat abdominal aorta transplantation, and the intervention group of tadarafil 0.5 mg/(kg Lewis rats received BN rat abdominal aorta transplantation. The transplanted arteries were harvested at 4 and 8 weeks after operation. The expression of PKG- 鈪，

本文编号：2027750